CBSE Class 12 Biology Principles and Processes of Bio Technology. CBSE issues sample papers every year for students for class 12 board exams. Students should solve the CBSE issued sample papers to understand the pattern of the question paper which will come in class 12 board exams this year. The sample papers have been provided with marking scheme. It’s always recommended to practice as many CBSE sample papers as possible before the board examinations. Sample papers should be always practiced in examination condition at home or school and the student should show the answers to teachers for checking or compare with the answers provided. Students can download the sample papers in pdf format free and score better marks in examinations. Refer to other links too for latest sample papers.
1 Name an enzyme catalysing the removal of nucleotides from the ends of DNA.
2 Write the Significance of 'heat shock' method in bacterial transformation.
3 Name two bacteria which are the sources of restriction endonuclease?
4 Identify the steps of PCR in which Taq polymerase is used.
5 Define recombinant protein.
6 What does ‘competent’ refer to in competent cells used in transformation experiments? Describe the role of CaCl2 in the preparation of competent cells?
7 What is the significance of adding proteases at the time of isolation of genetic material (DNA). Name the enzyme used to digest the cell wall of fungi.
8 What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector? Name the vector used to transfer gene of interest to animal cell.
9 How does one visualise DNA on an agarose gel?
10 For selection of recombinants, insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chormogenic substrate. Give reasons.
11 Describe the role of Agrobacterium tumefaciens in transforming a plant cell.
12 a) What are molecular scissors? Give one example. b) Explain their role in recombinant DNA technology.
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