CBSE Class 12 Bio Technology

Biotechnology is the most important subject in the Science stream, and our Class 12 Biotechnology page here has been designed to provide you all resources which can help you to get more marks in exams. The exam will have 50% Competency-Based questions. To get success you need to shift from rote learning to deep conceptual application of all concepts. There will be 70-mark theory paper and 30-mark practical assessment. Our teachers have preapred the below provided educational material which covers everything from Protein Engineering and Recombinant DNA Technology. Start your journey today with the most updated CBSE syllabus provided below and also get your seat in prestigious research and medical institutions.

click below for class 12 bio technology worksheets, assignments, syllabus, ncert cbse books, ncert solutions, hots, multiple choice questions (mcqs), easy to learn concepts and study notes of all class 12 bio technology chapters, online tests, value based questions (vbqs), sample papers and last year solved question papers

Important Practice Resources for Free Printable Worksheets PDF

The Biotechnology paper will be a blend of biology, technology, and analytical logic. To score high marks in exams students must focus on the higher order thinking skills (HOTS) that now have more weightage in the assessment structure. Our study material has been aligned with the NCERT approach. We have emphasised on Assertion-Reasoning and Source-Based Case Studies. We have provided simplified explanations of complex metabolic pathways and molecular biology techniques. We have provided detailed chapter-wise weightage and specialized mock tests. Be a high achiever by mastering the application of genetic engineering and bioinformatics through our result-oriented study material.

Unit V: Protein and Gene Manipulation (40 Marks)

  • Chapter-1: Recombinant DNA Technology: Introduction, Tool of Recombinant DNA technology, making rDNA molecule, Introduction of recombinant DNA into host cells, Identification of recombinants, Polymerase Chain Reaction (PCR), DNA Sequencing.
  • Chapter-2: Protein Structure and Engineering: Introduction to the world of proteins, Structure-function Relationship in proteins, Characterization of proteins, Protein based products, Designing proteins (Protein Engineering).
  • Chapter-3: Genomics, Proteomics and Bioinformatics: Gene prediction and counting, Genome similarity, SNPs and Comparative genomics, Functional genomics, Proteomics, Information sources, Analysis using bioinformatics tools.

Note for Students: This unit covers the fundamental tools and techniques of genetic engineering along with the structural analysis of proteins and the complex study of genomes and proteomes.

Unit VI: Cell Culture and Genetic Manipulation (30 Marks)

  • Chapter-1: Microbial Cell Culture and its Applications: Introduction, Microbial nutrition and culture techniques, Measurement and kinetics of microbial growth, Isolation of microbial products, Strain isolation and improvement, Applications of microbial culture technology.
  • Chapter-2: Plant Cell Culture and Applications: Introduction, Cell and tissue culture techniques, Applications of cell and tissue culture, Transgenic plants with beneficial traits, Biosafety of transgenic plants.
  • Chapter-3: Animal Cell Culture and Applications: Introduction, Animal cell culture techniques, Applications of animal cell culture, Stem cell technology.

Note for Students: This unit delves into the methodologies of culturing microbial, plant, and animal cells, highlighting their diverse applications in technology and medicine.

PRACTICALS (30 Marks)

  • Use of special equipment in biotechnology experiments
  • Isolation of bacterial plasmid DNA
  • Detection of DNA by gel electrophoresis
  • Estimation of DNA by UV spectroscopy
  • Isolation of bacteria from curd & staining of bacteria
  • Cell viability assay using Evan’s blue dye exclusion method
  • Data retrieval and database search using internet site NCBI and download a DNA and protein sequence from internet, analyze it and comment on it
  • Reading of a DNA sequencing gel to arrive at the sequence
  • Project work

Note for Students: Students are required to perform these experiments to gain hands-on experience in molecular techniques, microbial analysis, and bioinformatics data retrieval.

Scheme of Evaluation: Practicals (30 Marks)

  • Two experiments: 6+6 marks (only one computer based practical)
  • Practical record: 04 Marks
  • Viva on Practical: 04 Marks
  • Project work: 10 Marks (Write up: 05 Marks; Viva on project: 05 Marks)

Note for Students: The evaluation process emphasizes hands-on proficiency in experiments, the quality of the project report, and the student's ability to defend their work during the viva.

Assessment Areas - Theory (70 Marks)

  • Demonstrate, Knowledge and Understanding (50%): State, name, list, identify, define, suggest, describe, outline, summarize, etc.
  • Application of Knowledge / Concepts (30%): Calculate, illustrate, show, adapt, explain, distinguish, etc.
  • Analyse, Evaluate and Create (20%): Interpret, analyse, compare, contrast, examine, evaluate, discuss, construct, etc.

Note for Students: The theory examination will include a variety of question types such as MCQs, Assertion-Reasoning, and Case-based questions, with an internal choice of approximately 33%.