CBSE Class 12 Biology Molecular Basis of Inheritance Worksheet Set F

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Chapter 6 Molecular Basis of Inheritance Biology Worksheet for Class 12

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Class 12 Biology Chapter 6 Molecular Basis of Inheritance Worksheet Pdf

MOLECULAR BASIS OF INHERITANCE
 
DNA (Deoxyribonucleic Acid) and RNA (Ribonucleic Acid) are two types of nucleic acid found in living organisms. DNA acts as genetic material in most of the organisms. RNA also acts as genetic material in some organisms as in some viruses and acts as messenger. It functions as adapter, structural, and in some cases as a catalytic molecule
The DNA - it is a long polymer of deoxyribonucleotides. A pair of nucleotide is also known as base pairs. Length of DNA is usually defined as number of nucleotides present in it. Escherichia coli have 4.6 x 106 bp and haploid content of human DNA is 3.3 × 109 bp.
 
Structure of Polynucleotide Chain
CBSE Class 12 Biology Molecular Basis of Inheritance Worksheet 1
 
A nucleotide has three components – a nitrogenous base, a pentose sugar (ribose in case of RNA, and deoxyribose for DNA), and a phosphate group. There are two types of nitrogenous bases – Purines (Adenine and Guanine), and Pyrimidines (Cytosine, Uracil and Thymine).
 
Cytosine is common for both DNA and RNA and Thymine is present in DNA. Uracil is present in RNA at the place of Thymine.

CBSE Class 12 Biology Molecular Basis of Inheritance Worksheet 2

 
A polynucleotide chain
 
A nitrogenous base is linked to pentose sugar with N-glycosidic linkage to form to form a nucleoside. When phosphate group is linked 5’-OH of a nucleoside through phosphoester linkage nucleotide is formed. Two nucleotides are linked through 3’-5’ phosphodiester linkage to form dinucleotide. More nucleotide joins together to form polynucleotide. In RNA, nucleotide residue has additional –OH group present at 2’-position in ribose and uracil is found at the place of Thymine.

CBSE Class 12 Biology Molecular Basis of Inheritance Worksheet 3

CBSE Class 12 Biology Molecular Basis of Inheritance Worksheet 4

Double Helix Model for Structure of DNA-James Watson and Francis Crick, based on X-ray diffraction data produced by Wilkin and Rosalind proposed this model of DNA.
 
The silent features of this model are
a) DNA is made of two polynucleotide chains in which backbone is made up of sugarphosphate and bases projected inside it. 
b) Two chains have anti-parallel polarity. One 5’à3’ and with 3’à5’.
c) The bases in two strands are paired through H-bonds. Adenine and Thymine forms double hydrogen bond and Guanine and Cytosine forms triple hydrogen bonds.
d) Two chains are coiled in right handed fashion. The pitch of helix is 3.4 nm and roughly 10 bp in each turn.
e) The plane of one base pair stacks over the other in double helix to confer stability.
CBSE Class 12 Biology Molecular Basis of Inheritance Worksheet 5
 
Francis Crick proposed the Central dogma in molecular biology, which states that the genetic information flows from DNA -----> RNA ------> Protein.

CBSE Class 12 Biology Molecular Basis of Inheritance Worksheet 6

 

Long Answer Questions

Question. How is the translation of mRNA terminated? Explain.
Answer When the A-site of ribosome reaches a termination codon, which does not code for any amino acid, no charged tRNA binds to the A-site.
 Dissociation of polypeptide from ribosome takes place, which is catalysed by a ‘release factor’.
 There are three termination codons namely UGA, UAG and UAA.

Question. (a) A DNA segment has a total of 1000 nucleotides, out of which 240 of them are adenine containing nucleotides. How many pyrimidine bases this DNA segment possesses?
(b) Draw a diagrammatic sketch of a portion of DNA segment to support your answer.
Answer. (a) A = T, A = 240, hence T = 240
A + T = 240 + 240 = 480
So, G + C = 1000 – 480 = 520
G = C, so C 520/2 =260
So, pyrimidines = C + T
= 260 + 240
= 500
(b)

CBSE Class 12 Biology Molecular Basis of Inheritance Worksheet Set F

Question. (a) Explain DNA polymorphism as the basis of genetic mapping of human genome.
(b) State the role of VNTR in DNA fingerprinting. 
Answer. (a) Genetic polymorphism means occurrence of genetic material in more than one form. It is of  three major types, i.e., allelic, SNP and RFLP.
Allelic polymorphism: Allelic polymorphism occurs due to multiple alleles of a gene. Allele possess different mutations which alter the structure and function of a protein formed by them as a result, change in phenotype may occur.
SNP or single nucleotide polymorphism: SNP is very useful for locating alleles, identifying disease-associated sequence and tracing human history.
(b) Variable Number Tandem Repeats (VNTRs) are used in DNA fingerprinting as markers.
VNTRs vary from person to person and are inherited from one generation to the next.
Therefore, only closely related individuals have similar VNTRs.

Question. (a) Why did Hershey and Chase use radioactive sulphur and radioactive phosphorus in their experiment?
(b) Write the conclusion they arrived at and how.
Answer. • Procedure:
(i) Some bacteriophage virus were grown on a medium that contained radioactive phosphorus (32P) and some in another medium with radioactive sulphur (35S).
(ii) Viruses grown in the presence of radioactive phosphorus (32P) contained radioactive DNA.
(iii) Similar viruses grown in presence of radioactive sulphur (35S) contained radioactive protein.
(iv) Both the radioactive virus types were allowed to infect E. coli separately.
(v) Soon after infection, the bacterial cells were gently agitated in blender to remove viral coats from the bacteria.
(vi) The culture was also centrifuged to separate the viral particle from the bacterial cell.

Question. “A very small sample of tissue or even a drop of blood can help determine paternity”. Provide a scientific explanation to substantiate the statement. 
Answer. (i) DNA from all cells of an individual shows the same degree of polymorphism and therefore becomes a useful identification tool.
(ii) Polymorphs are heritable and the child inherits 50% of the chromosome from each parent.
(iii) With the help of PCR the small amount of DNA from blood can be amplified and be used in DNA finger printing to identitfhye paternity.

Question. (a) Identify strands ‘A’ and ‘B’ in the diagram of transcription unit given above and write the basis on which you identified them.
(b) Write the functions of RNA polymerase-I and RNA polymerase-III in eukaryotes.
Answer(a) A—Template strand
B—Coding strand
The templates are identified on the basis of polarity with respect to promoter. Template strand has polarity 3′ → 5′ and coding strand has polarity 5′ → 3′.
(b) RNA polymerase-I transcribes rRNAs.
RNA polymerase-III transcribes tRNA, 5srRNA and snRNA.

Question. State any two structural differences and one functional difference between DNA and rRNA.
Answer.

CBSE Class 12 Biology Molecular Basis of Inheritance Worksheet Set F

Question. DNA polymerase and RNA polymerase differ in their requirement while functioning Explain.
Answer

S.No. RNA polymerase DNA polymerase
(i) It cannot carry out proofreading. It carries out proofreading for DNA repair mechanism.
(ii) RNA polymerase does not require RNA primer for synthesis of RNA. DNA polymerase requires RNA primer for synthesis of DNA.
(iii) It uses ribonucleotides for RNA synthesis. It uses deoxyribonucleotides for DNA synthesis.

Question. The average length of a DNA double helix in a typical mammalian cell is approximately 2.2 metres and the dimension of the nucleus is about 10–6 m.
(a) How is it possible that long DNA polymers are packed within a very small nucleus?
(b) Differentiate between euchromatin and heterochromatin.
(c) Mention the role of non-histone chromosomal protein.

Answer. (a) Packaging of DNA in eukaryotes
 Roger Kornberg (1974) reported that chromosome is made up of DNA and protein.
 Later, Beadle and Tatum reported that chromatin fibres look like beads on the string, where beads are repeated units of proteins.
 The proteins associated with DNA are of two types—basic proteins (histones) and acidic non-histone chromosomal (NHC) proteins.
 The negatively charged DNA molecule wraps around the positively charged histone proteins to form a structure called nucleosome.
 The nucleosome core is made up of four types of histone proteins—H2A, H2B, H3 and H4 occurring in pairs.
(c) The packaging of chromatin at higher level requires the presence of non-histone chromosomal protein.

Question. List the criteria a molecule that can act as genetic material must fulfill. Which one of the criteria are best fulfilled by DNA or by RNA thus making one of them a better genetic material than the other? Explain. 
Answer. A molecule that can act as a genetic material must fulfill the following criteria:
(i) It should be able to generate its replica (Replication).
(ii) It should chemically and structurally be stable.
(iii) It should provide the scope for slow changes (mutation) that are required for evolution.
(iv) It should be able to express itself in the form of ‘Mendelian Characters’.
In DNA the two strands being complementary if separated by heating come together, when appropriate conditions are provided. Further, 2’-OH group present at every nucleotide in RNA is also now known to be catalytic, hence reactive. Therefore DNA chemically is less reactive and structurally more stable when compared to RNA. Therefore, among the two nucleic acids, the DNA is a better genetic material. The presence of thymine at the place of uracil also confers additional stability to DNA.
Both DNA and RNA are able to mutate. In fact, RNA being unstable, mutate at a faster rate. RNA can directly code for the synthesis of proteins, hence can easily express the characters. DNA, however, is dependent on RNA for synthesis of proteins. The protein synthesising machinery has evolved around RNA.

Question. (a) How are the following formed and involved in DNA packaging in a nucleus of a cell?
(i) Histone octomer
(ii) Nucleosome
(iii) Chromatin
(b) Differentiate between Euchromatin and Heterochromatin. 

Answer. (a) (i) Eight molecules of positively charged basic proteins called histones are organised to form histone octomer.
(ii) Negatively charged DNA is wrapped around positively charged histone octamer to give rise to nucleosome.
(iii) Nucleosome constitute the repeating unit of a structure called chromatin.
(b)

S.No. Euchromatin Heterochromatin
(i) Regions of chromatin, which are loosely packed during interphase are called euchromatin. Regions of chromatin, which are densely packed during cell division are called heterochromatin.
(ii) When chromosomes are stained with Feulgen stain (specific for DNA), these appear as lightly stained chromatin. When chromosomes are stained with Feulgen stain, these appear as intensely stained chromatin.
(iii) Euchromatin contains active genes. Heterochromatin contains inactive genes.
(iv) They do not contain repetitive DNA sequences. They are enriched with highly repetitive tandemly arranged DNA sequences. 
(v) It is transcriptionally active. It is transcriptionally inactive.

Question. What background information did Watson and Crick had available with them for developing a model of DNA? What was their own contribution?
Answer. Watson and Crick had the following informations which helped them to develop a model of DNA:
(i) Chargaff’s Law suggesting A=T and C G.
(ii) Wilkins and Franklin’s X-ray diffraction studies on DNA’s physical structure.
Based on these information, Watson and crick proposed
(i) complementary base-pairing of nitrogenous bases
(ii) semi-conservative mode of replication
(iii) occurrence of mutation through tautomerism.

Q. 5. Describe the packaging of DNA helix in a prokaryotic cell and an eukaryotic nucleus.
Answer. (i) Packaging of DNA in prokaryotes
 In prokaryotes, well-defined nucleus is absent so DNA is present in a region called nucleoid.
The negatively charged DNA is coiled with some positively charged non-histone basic proteins.
 DNA in nucleoid is organised in large loops held by proteins.
(ii) Packaging of DNA in eukaryotes
 Roger Kornberg (1974) reported that chromosome is made up of DNA and protein.
 Later, Beadle and Tatum reported that chromatin fibres look like beads on the string, where beads are repeated units of proteins.
 The proteins associated with DNA are of two types—basic proteins (histones) and acidic non-histone chromosomal (NHC) proteins.
 The negatively charged DNA molecule wraps around the positively charged histone proteins to form a structure called nucleosome.

Question. (a) How did Griffith explain the transformation of R-strain (non-virulent) bacteria into S-strain (virulent)?
(b) Explain how MacLeod, McCarty and Avery determined the biochemical nature of the molecule responsible for transforming R-strain bacteria into S-strain bacteria.
                                                  ​​​​​​​                         ​​​​​​​                         ​​OR
(a) Describe the various steps of Griffith’s experiment that led to the conclusion of the ‘Transforming Principle’.
(b) How did the chemical nature of the ‘Transforming Principle’ get established?

Answer. (a)  Frederick Griffith (1928) conducted experiments with Streptococcus pneumoniae (bacterium causing pneumonia).
 He observed two strains of this bacterium—one forming smooth shiny colonies (S-type) with capsule,while other forming rough colonies (R-type) without capsule.
(b)  Oswald Avery, Colin MacLeod and Maclyn McCarty repeated Griffith’s experiment in an in vitro system in order to determine biochemical nature of transforming principle.
 They purified biochemicals (proteins, DNA, RNA) from heat-killed S-type cells, and checked which of these could transform live R-type cells into S-type cell. They observed that DNA alone from S-type cells caused transformation of R-type cells into virulent S-type cells.
 They also discovered that proteases (protein digesting enzymes) and RNases (RNA digesting enzymes) did not affect transformation while DNases inhibited the process.
 They concluded that DNA is the hereditary material.

Question. (a) Write the scientific name of the bacterium used by Frederick Griffith in his experiment.
(b) How did he prove that some ‘transforming principle’ is responsible for transformation of the non-virulent strains of bacteria into the virulent form?
(c) State the biochemical nature of ‘transforming principle’.
(d) Name the scientists who proved it. 
Answer. (a) Streptococcus pneumoniae
(b) Refer to Basic Concepts Point 6.
(c) ‘The transforming principle’ was nucleic acid, i.e., DNA.
(d) It was proved by O. Avery, C. MacLeod and M. McCarty.

Question. (a) Explain the experiment performed by Griffith on Streptococcus pneumoniae. What did he conclude from this experiment? 
(b) Name the three scientists who followed up Griffith’s experiments.
(c) What did they conclude and how?
Answer. (a) Refer to Basic Concepts Point 5.
(b) Oswald Avery, Colin MacLeod and Maclyn McCarty.
(c)  Oswald Avery, Colin MacLeod and Maclyn McCarty repeated Griffith’s experiment in an in vitro system in order to determine biochemical nature of transforming principle.
 They purified biochemicals (proteins, DNA, RNA) from heat-killed S-type cells, and checked which of these could transform live R-type cells into S-type cell. They observed that DNA alone from S-type cells caused transformation of R-type cells into virulent S-type cells.
 They also discovered that proteases (protein digesting enzymes) and RNases (RNA digesting enzymes) did not affect transformation while DNases inhibited the process.
 They concluded that DNA is the hereditary material.

Question. (a) What did Meselson and Stahl observe when
(i) they cultured E. coli in a medium containing 15NH4Cl for a few generations and centrifuged the content?
(ii) they transferred one such bacterium to the normal medium of NH4Cl and cultured for 2 generations?
(b) What did Meselson and Stahl conclude from this experiment? Explain with the help of diagrams.
(c) Which is the first genetic material? Give reasons in support of your answer.

Answer. (a) (i) Meselson and Stahl observed that in the E. coli bacterium the DNA becomes completely labelled with 15N medium after few generations.
(ii) After two generations, they observed that density changed and showed equal amount of light DNA (14N) and dark hybrid DNA (15N–14N).
(b) They concluded that DNA replicates semi-conservatively.

CBSE Class 12 Biology Molecular Basis of Inheritance Worksheet Set F

(c) RNA is the first genetic material.
Reasons:
(i) RNA is highly reactive and acts as a catalyst as well as a genetic material.
(ii) Essential life processes such as metabolism, translation and splicing evolved around RNA.
(iii) It expresses itself through proteins.

Question. Describe the Hershey−Chase experiment. Write the conclusion they arrived at after the experiment.
                         ​​​​​​​                         ​​​​​​​                         ​​​​​​​OR
How did Hershey and Chase established that DNA is transferred from virus to bacteria?
Answer. In some viruses, RNA is the genetic material (e.g., Tobacco Mosaic virus). RNA also performs functions of messenger and adapter.
(i) DNA and RNA have the ability to direct their duplications because of rule of base pairing and complementarity but proteins fail to fulfill first criteria itself.
(ii) Genetic material should be stable so as not to change with different stages of life cycle, age or change in physiology of organism.
(iii) RNA being unstable mutates at a faster rate. Thus, viruses having RNA genome and having shorter life span mutate and evolve faster.
(iv) RNA can code directly for protein synthesis and hence can easily express characters. But DNA is dependent on RNA for protein synthesis. Protein synthesizing machinery has evolved around RNA.

Question. You are repeating the Hershey–Chase experiment and are provided with two isotopes: 32P and 15N (in place of 35S in the original experiment). How do you expect your results to be different? 
Answer. Use of 15N will be inappropriate because method of detection of 35P and 15N is different (32Pbeing a radioactive isotope while 15N is not radioactive but is the heavier isotope of nitrogen).
Even if 15N was radioactive then its presence would have been detected both inside the cell (l5N incorporated as nitrogenous base in DNA) as well as in the supernatant because 15N would also get incorporated in amino group of amino acids in proteins). Hence, the use of 15N would not give any conclusive results.

Question. A criminal blew himself up in a local market when was chased by cops. His face was beyond recognition. Suggest and describe a modern technique that can help establish his identity.
Answer. The identity can be established by the technique of DNA fingerprinting.
For method:  Dr. Alec Jeffreys developed the technique of DNA fingerprinting in an attempt to identify DNA marker for inherited diseases.
 Human genome has 3 × 109 bp. 99.9% of base sequences among humans are the same, which makes every individual unique in phenotype.
 Polymorphism: The genome consists of small stretches of DNA which are repeated many times.
These are called repetitive DNA and comprise of satellite DNA. Satellite DNA does not code for any proteins but form large portion of human genome. These sequences show high degree of polymorphism. As polymorphisms are inheritable from parents to children, DNA fingerprinting is the basis of paternity testing. Polymorphism arises due to mutations. New mutations may arise in somatic cells or in germ cells. If mutation occurs in germ cells; it is passed on to offsprings. If an inheritable mutation is observed in a population at high frequency, it is
called as DNA polymorphism. Polymorphism ranges from single nucleotide change to very large scale changes.

Question. Answer the following questions based on Hershey and Chases’s experiments:
(a) Name the kind of virus they worked with and why.
(b) Why did they use two types of culture media to grow viruses in? Explain.
(c) What was the need for using a blender and later a centrifuge during their experiments?
(d) State the conclusion drawn by them after the experiments.

Answer. (a) They worked with bacteriophage because when it attacks a bacteria it only inserts its genetic material in its body.
(b) They grew some viruses on a medium that contained radioactive phosphorus and some others on medium that contained radioactive sulphur. Viruses grown in the presence of radioactive phosphorus contained radioactive DNA but not radioactive protein because DNA contains phosphorus but protein does not. Similarly, viruses grown on radioactive sulphur contained radioactive protein but not radioactive DNA because DNA does not contain sulphur.
(c) Blender was used to agitate the bacteria to remove the viral coats from them. Centrifuge was used to separate virus particle from the bacteria.
(d) Bacteria which was infected with viruses that had radioactive DNA were radioactive, indicating that DNA was the material that passed from the virus to the bacteria. Bacteria that
were infected with viruses that had radioactive proteins were not radioactive. This indicates that proteins did not enter the bacteria from the viruses. DNA is therefore the genetic material that is passed from virus to bacteria.

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