CBSE Class 12 Biotechnology Sample Paper 2019 Solved

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Read and download the CBSE Class 12 Biotechnology Sample Paper 2019 Solved, designed by expert teachers according to the latest 2026-27 curriculum and examination pattern issued by CBSE, NCERT, and KVS. These Class 12 Bio Technology sample papers include detailed step-by-step solutions, helping students to practice and evaluate their performance against the most recent marking schemes.

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Check out our Class 12 Bio Technology model papers to get familiar with expected questions and chapter weightage. Practicing these guess papers regularly improves your speed and fixes weak concepts. You can also explore our historical Class 12 Bio Technology Question Papers to boost your confidence further.

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CBSE Class 12 Biotechnology Sample Paper 2019 Solved. CBSE issues sample papers every year for students for class 12 board exams. Students should solve the CBSE issued sample papers to understand the pattern of the question paper which will come in class 12 board exams this year. The sample papers have been provided with marking scheme. It’s always recommended to practice as many CBSE sample papers as possible before the board examinations. Sample papers should be always practiced in examination condition at home or school and the student should show the answers to teachers for checking or compare with the answers provided. Students can download the sample papers in pdf format free and score better marks in examinations. Refer to other links too for latest sample papers.

1. 4 copies of ds DNA are subjected to polymerase chain reaction. How many copies would be obtained after 20 cycles?

OR

How can we use LEU 2 gene as a selectable marker?

2. Specify the role of alkaline phosphatase in cloning. 

3. Specific activity increases during subsequent steps of a protein purification scheme. How can you relate it with the purity of protein?

4. Animal cells in a culture medium were placed in a regular incubator used for growing bacterial cells. Do you expect the animal cells to grow or not?

5. What is Gene Knock out? 

6. What will be the consequence if a protein is having an altered structure?

OR

Expand and define PER?

SECTION-B

7. What are inverted microscopes and why are they useful in animal cell culture lab?

OR

Enlist the advantages and limitations of animal cell culture (two each).

8. Explain a visual method of screening the transformed host cells. 
 
9. Interspecific cross leads to formation of sterile seeds. What could be the reasons for the same and how can normal development be achieved?
 
10. A protein from cell A and cell B is compared to find the whole protein pattern by a technique
developed by O’Farrel. Name the technique. State its principle.
 
11. C.elegans is a eukaryotic organism with a genome of 97 Mb and about 20,000 genes. What organizational features of this genome are unusual when compared to the genomes of other eukaryotes, such as yeast and Drosophila?
 
OR
Annotation of human genome sequence reveals that our genome contains 30000- 33000 genes.Proteomic analysis indicates that human cells are capable of synthesizing more than 30,000 different proteins. How can this discrepancy be reconciled?
 
12. Foot and Mouth Disease Virus(FMDV) vaccine is made by growing the virus in animal cells,harvesting the virus and inactivating it for vaccine formulations. Given the following data,calculate the weight and volume of the harvested virus from a bioreactor-
(a) Total bioreactor capacity= 1000 l( atleast 20% space must be kept for oxygen and CO2 )
(b) Number of animal cells= 105/ml
(c) Number of virus particles/animal cell=50
Assume the virus to be a sphere with a gram molecular weight of 106 Daltons (1 million) and radius = 1nm.
OR
Recombinant insulin is produced at 100 mg/L by E. coli at a cell concentration of 1 g/L.
Calculate the volume of reactor (size of the fermentor) needed to produce 1 Kilogram of insulin in the following conditions:
(a) When the cell concentration is 1 g/L and insulin production is 100 mg /L.
(b) When the cell concentration is 50 g/L and insulin production is 100 mg /L.
 
13. Name the plant variations developed by long term callus and suspension culture in plants.How can such variations be used in crop improvement?
 
14. Foaming is a problem in most microbiological processes. Mention any two possible causes of this problem? How can it be controlled?
 
SECTION-C
 
15. What is Molecular Pharming ?State its advantages (any 4) 
 
16. Outline the process of creation of chimeric mouse by embryonic stem cell culture.


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