SECTION A
1. Why is agar added to the culture medium used for culturing microbes ?
2. Pectinase enzyme is needed to isolate single cells from a plant tissue. Why ?
OR
For genetic transformation vector, which two DNA sequences are cloned in the T-DNA region of Ti-plasmid in place of unwanted DNA sequences ?
3. Give an example of a disease caused by the absence of the enzyme adenosine deaminase.
OR
What is the cause of thalassemia ?
4. In recombinant DNA technology, what is an ‘insert’ ?
5. Which database retrieval tool carries information on taxonomic classification of an organism ?
OR
Name a single gene mutation in humans which follows Mendelian inheritance, having chromosome 7 as its genomic location.
6. Frozen animal cell culture should be thawed in a water-bath maintained at 370C, with moderate shaking. Why ?
7. What are the two limitations of the germplasm conservation through conventional methods ?
8. Polyethylene glycol is a
(A) Fusogenic chemical
(B) Callus stimulant
(C) Electrofusion stimulant
(D) Differentiation stimulant
9. Which one of the following restriction enzymes produces blunt ends in a DNA fragment ?
(A) Alu I
(B) Eco RI
(C) Bam HI
(D) Hind III
10. The study of full complement of proteins expressed by a genome is called
(A) Proteome
(B) Proteomics
(C) Genomics
(D) Protein analysis
11. Which of the following is a function of cryoprotective agents ?
(A) Long-term preservation of cultures
(B) Prevention of cell damage due to ice crystal formation
(C) Prevent formation of inclusion bodies
(D) To trap the liquid nitrogen
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