CBSE Class 12 Biology Biotechnology Principles and Processes MCQs Set D

Refer to CBSE Class 12 Biology Biotechnology Principles and Processes MCQs Set D provided below available for download in Pdf. The MCQ Questions for Class 12 Biology with answers are aligned as per the latest syllabus and exam pattern suggested by CBSE, NCERT and KVS. Chapter 9 Biotechnology Principles and Processes Class 12 MCQ are an important part of exams for Class 12 Biology and if practiced properly can help you to improve your understanding and get higher marks. Refer to more Chapter-wise MCQs for CBSE Class 12 Biology and also download more latest study material for all subjects

MCQ for Class 12 Biology Chapter 9 Biotechnology Principles and Processes

Class 12 Biology students should refer to the following multiple-choice questions with answers for Chapter 9 Biotechnology Principles and Processes in Class 12.

Chapter 9 Biotechnology Principles and Processes MCQ Questions Class 12 Biology with Answers

Question : ‘Restriction’ in Restriction enzyme refers to
(A) cleaving of phosphodiester bond in DNA by the enzyme.
(B) cutting of DNA at specific position only.
(C) prevention of the multiplication of bacteriophage in bacteria.
(D) All of the above. 

Answer : B

Question : While isolating DNA from bacteria, which of the following enzymes is not used?
(A) Lysozyme
(B) Ribonuclease
(C) Deoxyribonuclease
(D) Protease

Answer : C

Question : A biotechnologist wanted to create a colony of E.coli possessing the plasmid pBR322, sensitive to Tetracycline. Which one of the following restriction sites would he use to ligate a foreign DNA?
(A) Sal I
(B) Pvu I
(C) EcoRI
(D) Hind III

Answer : A

Question : The correct order of step in polymerase chain reaction (PCR) is :
(A) Extension, Denaturation, Annealing
(B) Denaturation, Annealing, Extension
(C) Denaturation, Extension, Annealing
(D) Annealing, Extension, Denaturation. 

Answer : B

Question : The transfer of genetic material from one bacterium to another through the mediation of a vector like virus is termed as
(A) transduction
(B) conjugation
(C) transformation
(D) translation 

Answer : A

Question : Which of the following bacteria is not a source of restriction endonuclease?
(A) Haemophilus influenzae
(B) Escherichia coli
(C) Agrobacterium tumefaciens
(D) Bacillus amyloli 

Answer : C

Question : What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?
(A) The larger the fragment size, farther it moves.
(B) The smaller the fragment size, farther it moves.
(C) Positively charged fragment move to farther end.
(D) Negatively charged fragment do not move.

Answer : B

Question : An antibiotic resistance gene in a vector usually helps in the selection of
(A) Competent cells
(B) Transformed cells
(C) Recombinant cells
(D) None of the above

Answer : B

Question : The process of separation and purification of expressed protein before marketing is called :
(A) Upstream processing
(B) Downstream processing
(C) Bio processing
(D) Post production processing. 

Answer : B

Question : Significance of ‘heat shock’ method in bacterial transformation is to facilitate
(A) Binding of DNA to the cell wall.
(B) Uptake of DNA through membrane transport proteins.
(C) Uptake of DNA through transient pores in the bacterial cell wall.
(D) Expression of antibiotic resistance gene.

Answer : C

Question : An enzyme catalysing the removal of nucleotides from the ends of DNA is
(A) endonuclease
(B) exonuclease
(C) DNA ligase.
(D) Hind – II. 

Answer : B

Question : Stirred-tank bioreactors have been designed for.
(A) Ensuring anaerobic conditions in culture vessel
(B) Purification of product
(C) Addition of preservatives to product
(D) Availability of oxygen throughout process.

Answer : D

Question : The restriction enzymes are used in genetic engineering, because 
a) they can degrade harmful proteins
b) they can join different DNA fragments
c) they can cut DNA at spe cific base sequence
d) they are nucleases that cut DNA at variable sites

Answer :  A

Question : The basis for DNA fingerprinting is  
a) occurrence of Restriction Fragment Length Polymorphism (RFLP)
b) phe noty pic   differences  between individuals
c) availability of cloned DNA
d) knowledge of human karyotype

Answer :  A

Question : Introduction of one or more genes into an organism which normally does not possess them or their deletion by using artificial means (not by breeding) comes under 
a) Molecular Biology
b) Cytogenetics
c) Genetic hybridization
d) Genetic Engineering

Answer :  D

Question : Recombinant DNA is obtained by cleaving the pro-DNA by                                   
a) primase
b) exonucleases
c) ligase
d) restriction endonuclease

Answer :  D

Question : Genetic engineering is possible, because
a) the phenomenon of transduction in bacteria is well understood
b) we can see DNA by electron microscope
c) we can cut DNA at specific sites by endonucleases like DNAse-I
d) restriction endonucleases purified from bacteria can be used in vitro

Answer :  D

Question : The process of replication in plasmid DNA, other than initiation, is controlled by 
a) mitochondrial gene
b) bacterial gene
c) plasmid gene   
d) None of the above

Answer :  B

Question : Which of the following is related to genetic engineering?               
a) Mutation             
b)  Plasmid
c) Plastid               
d) Heterosis

Answer :  B

Question : Plasmids are suitable vectors for gene clon- ing because  
a) these are small circular DNA molecules which can integrate with host chromo- somal DNA
b) these are small circular DNA molecules with their own replication origin site
c) these can shuttle between prokaryotic and eukaryotic cells
d) these often carry antibiotic resistance genes 

Answer :  B

Question : Maximum number of bases in plasmids dis- covered so far is            
a) 50 kilo base       
b)  500 kilo base
c) 5000 kilo base   
d) 5 kilo base

Answer :  B

Question : Plasmid is                                                 
a) fragment of DNA which acts as vector
b) a fragment which joins two genes
c) mRNA which acts as carrier
d) autotrophic fragment

Answer :  A

Question : In bacteria, plasmid is                            
a) extrachromosomal material
b) main DNA
c) non-functional DNA
d) repetetive gene

 Answer :  A

Question : DNA finger-printing refers to                     
a) molecular analysis or profiles of DNA samples
b) analysis of DNA samples using imprint- ing device
c) techniques used for molecular analysis of different specimens of DNA
d) techniques used for identification of finger prints of individuals

 Answer :  A

Question : Restriction endonudeases                      
a) are present in mammalian cells for degradation of DNA when the cell dies
b) are used in genetic engineering for ligating two DNA molecules
c) are used for in vitro DNA synthesis
d) are synthesized by bacteria as part of their defence mechanism

Answer :  A

Question : Which one of the following is commonly used in transfer of foreign DNA into crop plants?                                                   
a) Agrobacterium tumefaciens
b) Penicillium expansum
c) Trichoderma harzianum
d) Meloidogyne incognita

Answer :  A

Question : Which one of the following is used as vector for cloning genes into higher organisms?
a) Baculovirus                   
b) Salmonella typhimurium
c) Rhizopus nigricans
d) Retrovirus

Answer :  D

Question : DNA or RNA segment tagged with a radio- active molecule is called:            
a) Vector               
b)  Probe
c) Clone               
d) Plasmid

Answer :  B

Question : Restriction endonucleases are enzymes which                                          
a) make cuts at specific positions within the DNA molecule
b) recognize a specific nucleotide sequence for binding of DNA ligase
c) restrict the action of the enzyme DNA polymerase
d) remove nucleotides from the ends of the DNA molecule

Answer :  A

Question : Satellite DNA is useful tool in       
a) Organ transplantation
b) Sex determination
c) Forensic science
d) Genetic engineering

Answer :  D

Question : Silencing of mRNA has been used in pro- ducing transgenic plants resistant to
a) Bacterial blights
b) Bollworms
c) Nematodes
d) White rusts

Answer :  C

Question : Which one of the following techniques made it possible to genetically engineer living or- ganisms                                    
a) Hybridization
b) Recombinant DNA techniques
c) X-ray diffraction
d) Heavier isotope labelling

Answer :  B

Question : There is a restriction endonuclease called EcoRI. What does “co” part in it stand for ? 
a) Colon               
b)  Coelom
c) Coenzyme       
d) Coli  

Answer :  D

Question : In genetic engineering, the antibiotics are used         
a) To select healthy vectors
b) As sequences from where replication starts
c) To keep the cultures free of infection
d) As selectable markers

Answer :  D

Question : Which one of the following represents a pal- indromic sequence in DNA ? 
a) 5'-CCAATG-3' 3'-GAATCC-5'
b) 5'-CATTAG-3' 3'-GATAAC-5'
c) 5'-GATACC-3' 3'-CCTAAG-5'
d) 5'-GAATTC-3' 3'-CTTAAG-5'

Answer :  D

Question : What is it that forms the basis of DNA Fin- gerprinting ?             
a) The relative difference in the DNA occurrence in blood, skin and saliva
b) The relative amount of DNA in the ridges and grooves of the fingerprints
c) S ate llite DNA occur r ing a s highly repeated short DNA segments
d) The relative proportions of purines and pyrimidines in DNA

Answer :  C

Question : Biolistics (gene-gun) is suitable for
a) Transformation of plant cells
b) Constructing recombinant DNA by joining with vectors
c) DNA finger printing
d) Disarming pathogen vectors

Answer :  A

MCQs for Chapter 9 Biotechnology Principles and Processes Biology Class 12

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