CBSE Class 12 Biology Biotechnology and Its Applications MCQs Set H

Question: The first human hormone produced by recombinant DNA technology is
a) insulin
b) estrogen
c) thyroxin
d) progesterone
Answer: a

Question: Which one of the following is not a GMO?
a) Bt brinjal
b) Golden rice
c) Tracy
d) Dolly
Answer: d

Question: Bt toxin kills the larvae of certain insects
a) by binding of activated toxin on mid gut epithelial cells, creating pores, leading to swelling and lysis.
b) by stopping transcription of larval cells.
c) by altering central dogma taking place in the cells of gut of larva.
d) by stopping protein synthesis.
Answer: a

Question: cryIIAb and cryIAb produce toxins that control
a) cotton bollworms and corn borer respectively
b) corn borer and cotton bollworms respectively
c) tobacco budworms and nematodes respectively
d) nematodes and tobacco budworms respectively
Answer: a

Question: How many recombinant therapeutics have been used for human diseases throughout the world?
a) 12
b) 24
c) 30
d) 56
Answer: c

Question: Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?
a) Retrovirus
b) Ti plasmid
c) λ phage
d) pBR322
Answer: a

Question: Which one of the following is now being commercially produced by biotechnological procedures?
a) Nicotine
b) Morphine
c) Quinine
d) Insulin
Answer: d

Question: Which one of the following vectors is used to replace the defective gene in gene therapy?
a) Adenovirus
b) Cosmid
c) Ri plasmid
d) Ti plasmid
Answer: a

Question: Which of the following statements (i – v) is/are incorrect ?
(i) Recombinant DNA technology is used to improve crop plants by increasing their productivity, by making them more nutritious and by developing disease resistant.
(ii) Bt cotton is resistant to bollworm infestation.
(iii) Bacillus thuringiensis can form cry protein during any phase of their growth.
(iv) Bacillus thuringiensis is not harmed by self cry protein because of its occurrence as protoxin (inactive).
(v) Protoxin cry protein is changed into active cry protein in the stomach of insects due to alkaline pH in stomach.
(a) Only (iii)
(b) (i) and (iv)
(c) All of these
(d) None of these
Answer: c

Question: Today, transgenic models exist for many human diseases which includes
(i) Cancer
(ii) Cystic fibrosis
(iii) Rheumatoid arthritis
(iv) Alzhiemer’s disease
(a) (i) and (iii) only
(b) (ii) and (iii) only
(c) (i), (ii) and (iii) only
(d) all of these
Answer: d

Question: Read the following four statements (A-D) about certain mistakes in two of them.
(A) The first transgenic buffalo, Rosie produced milk which was human alpha-lactalbumin enriched.
(B) Restriction enzymes are used in isolation of DNA from other macromolecules.
(C) Downstream processing is one of the steps of rDNA technology.
(D) Disarmed pathogen vectors are also used in transfer of rDNA into the host.
Which of the two statements have mistakes?
(a) B and C
(b) C and D
(c) A and C
(d) A and B
Answer: d

Question: Read the following four statements (i-iv) and answer the question ?
(i) The first transgenic buffalo, Rosie produced milk which was human alpha-lactal albumin enriched.
(ii) Restriction enzymes are used in isolation of DNA from other macro-molecules.
(iii) Downstream processing is one of the steps of RDNA technology.
(iv) Disarmed pathogen vectors are also used in transfer of R-DNA into the host.
Which are the two statements having mistakes?
(a) (ii) and (iii)
(b) (iii) and (iv)
(c) (i) and (iii)
(d) (i) and (ii)
Answer: d

Question: Transgenic animals are produced
(i) to study how genes are regulated and how they affect the normal functions of body and its development.
(ii) to study diseases.
(iii) to obtain useful biological products .
(iv) to test vaccine safety and chemical safety.
(a) (i), (ii), (iii) and (iv)
(b) (i) and (iv)
(c) (ii) and (iv)
(d) Only (i)
Answer: a

Question: The given statements are the steps in one type of gene therapy.
(i) Inject engineered cells into patients bone marrow.
(ii) Viral DNA carring the normal allele inserts into chromosome.
(iii) Let retrovirus infect bone marrow cells that have removed from patient and cultured.
(iv) Insert RNA version of normal allele into retrovirus.
The correct sequence is
(a) (i), (ii), (iii) and (iv)
(b) (iv), (iii), (ii) and (i)
(c) (i), (ii), (iv) and (iii)
(d) (iv), (iii), (i) and (ii)
Answer: b 

Question: Read the following statements and choose the correct statements.
(i) Gene therapy has been tested on a large number of patients with a wider variety of inherited genetic disorders, and in numerous cases it has produced a complete cure.
(ii) Genetic engineering has been used to produce insulin for curing the diabetes.
(iii) DNA hybridization is the base pairing of DNA from two different sources.
(iv) Genetic engineering is a technique of plant breeding.
(a) (i) and (ii)
(b) (ii) and (iii)
(c) (i), (ii) and (iii)
(d) All of these
Answer: b

Question: Match column-I with column-II and identify the correct option.
Column – I                          Column – II
A. Gene therapy               I. Rhizobium
B. Biofertilizer                   II. Cry gene
C. Bt cotton                     III. SCID
D. Humulin                      IV. Diabetes
(a) A – II; B – I; C – IV; D – III
(b) A – III; B – I; C – II; D – IV
(c) A – II; B – III; C – I; D – IV
(d) A – IV; B – I; C – II; D – III
Answer: b

Question: Match the items given in column – I with those in column – II and choose the correct option.
Column – I                             Column – II
A. GMO                               I. Increased shelf life
B. Flavr – Savr tomato       II. Bioresources
C. Biopiracy                       III. rDNA
D. E.coli                             IV. Insulin
(a) A – III; B – I; C – II; D – IV
(b) A – II; B – I; C – III; D – IV
(c) A – II; B – III; C – I; D – IV
(d) A – IV; B – I; C – II; D – III
Answer: a

Question: Which of the following statement(s) is/are incorrect?
(i) Insulin was originally extracted from pancreas of slaughtered pigs and cattle.
(ii) Animal insulin is difficult to obtain.
(iii) Animal insulin is identical to human insulin.
(iv) Non-human insulin caused some patients to develop allergy.
(v) Recombinant insulin is actually obtained from E. coli in bacterial cell.
(a) Only (i) and (ii)
(b) Only (iii) and (iv)
(c) Only (iii)
(d) Only (v)
Answer: c

Question: Match the following and choose the correct combination from the options given below :
Column – I                                   Column – II
A. Escherichia coli                       I. nif gene
B. Bacillus thuringiensis              II. Interferon
C. Rhizobium meliloti                  III. Bt toxin
D. Agrobacterium tumefaciens    IV. Vector
(a) A – II; B – I; C – (IV); D – III
(b) A – II; B – I; C – (III); D – IV
(c) A – II; B – III; C – I; D – IV
(d) A – IV; B – I; C – II; D – III
Answer: c

Question: Match column-I with column-II and choose the correct option.
Column – I                     Column – II
A. Golden Rice           I. Cry protein
B. Bt toxin                 II. Rich in vitamin A
C. RNAi                     III. First trangenic cow
D. Rosie                    IV. Gene silencing
(a) A – II; B – I; C – IV; D – III
(b) A – II; B – I; C – III; D – IV
(c) A – II; B – III; C – I; D – IV
(d) A – IV; B – I; C – II; D – III
Answer: a

Question: When a patient with defective ADA was treated, which of the following steps was performed for gene therapy?
(i) Lymphocytes were obtained from the patients.
(ii) Lymphocytes are transferred to culture dishes.
(iii) Lymphocytes were transfected with normal ADA genes.
(iv) The transfected cells are returned to the patients.
(a) All of these
(b) (iii) and (iv)
(c) Only (iv)
(d) SCID cannot be treated
Answer: a

Assertion and Reason Based MCQs

Directions : In the following questions a statement of assertion (a) is followed by a statement of reason (R). Mark the correct choice as :
(a) Both assertion (a) and reason (R) are true and reason (R) is the correct explanation of assertion (a).
(b) Both assertion (a) and reason (R) are true but reason (R) is not the correct explanation of assertion (a).
(c) Assertion (a) is true but reason (R) is false.
(d) Assertion (a) is false but reason (R) is true.

Question: Assertion (a) : Western ghats of India are one of the hot-spots of biodiversity.
Reason (R) : Western ghats have greater amphibian diversity than eastern ghats.
Answer: b

Question: Assertion (a) : Biologists are not sure about total number of prokaryotic species
Reason (R) : Nearly 45,000 species of plants and twice as many of animals have been recorded from India
Answer: c

Question: Assertion (a) : Sacred groves are highly protected.
Reason (R) : They are of religious importance to the communities.
Answer: a

Question: Assertion (a) : There is more species biodiversity in tropical latitudes than in temperate ones.
Reason (R) : Tropical environments, unlike temperate ones, are less seasonal, relatively more constant and predictable.
Answer: a

Question: Assertion (a) : Process of extinction is random and fast.
Reason (R) : Any species which can not adapt itself according to the environment, it cannot survive.
Answer: d

Question: Assertion (a) : Loss of habitat is the main cause of loss of biodiversity.
Reason (R) : This causes the increase in edge area and reduction in core area.
Answer: c

Question: Assertion (a) : Biodiversity means diversity at species level.
Reason (R) : Biodiversity is a diversity not only at species level but at all levels of biological organization.
Answer: d

Question: Assertion (a) : Biosphere reserves are also included under the ex-situ conservation strategies.
Reason (R) : Cropping and grazing are allowed in the transition zone of biosphere reserve.
Answer: d

Question: Assertion (a) : Many species like Stellar’s sea cow, passenger pigeon, etc., became extinct due to over exploitation.
Reason(R) : Over exploitation is a major cause of biodiversity loss.
Answer: b

Question: Assertion (a) : Percentage of animals is more than the percentage of plants.
Reason (R) : 70% of all the species are animals, while plants contribute nearly 22% of the total.
Answer: a