CBSE Class 12 Biology Biotechnology and Its Applications MCQs Set I

Question: Which variety of rice was patented by a U.S. company even though the highest number of its varieties is found in India?
a) Sharbati Sonara
b) Co-667
c) Basmati
d) Lerma Roja
Answer: c

Question: GEAC stands for
a) Genome Engineering Action Committee
b) Ground Environment Action Committee
c) Genetic Engineering Approval Committee
d) Genetic and Environment Approval Committee
Answer: c

Question: Bacillus thuringiensis (Bt) strains have been used for designing novel
a) bio-fertilizers
b) bio-metallurgical techniques
c) bio-mineralization processes
d) bio-insecticidal plants
Answer: d

Question: The transgenic animals are those which have
a) foreign DNA in some cells.
b) foreign DNA in all of their cells.
c) foreign RNA in all of their cells.
d) both (a) and (c)
Answer: b

Question: Cry-genes have been introduced in
a) cotton and corn
b) rice
c) potato and soyabean
d) all of the above
Answer: d

Question: In some children, ADA deficiency can be cured by
a) bone marrow transplantation
b) enzyme replacement therapy
c) both (a) and (b)
d) none of the above
Answer: c

Question: C-peptide of human insulin is
a) a part of mature insulin molecule.
b) responsible for formation of disulphide bridges.
c) removed during maturation of pro-insulin to insulin.
d) responsible for its biological activity.
Answer: c

Question: Bt toxin kills larvae of certain insects
a) by binding to midgut epithelial cells, creating pores leading to lysis
b) by stopping transcription
c) by altering central dogma
d) by stopping protein synthesis
Answer: a

Question: Genes of interest can be selected from a genomic library by using
a) restriction enzymes
b) cloning vectors
c) DNA probes
d) gene targets
Answer: c

Question: The protein α-1 antitrypsin is used to treat
a) cancer
b) rheumatoid arthritis
c) Alzheimer’s disease
d) emphysema
Answer: d

Question: E. coli are used in production of
a) rifampicin
b) LH
c) ecdysone
d) interferon
Answer: d

Question: RNA interference protects plants from nematodes by silencing gene using
a) dsDNA
b) ssDNA
c) dsRNA
d) target proteins
Answer: c

Question: The use of bioresources by multinational companies without authorization and compensation is called
a) bioethics
b) biopiracy
c) bioterror
d) bioweapon
Answer: b

Statement Type Questions

Question: Which of the following statement is correct about Bt toxin?
a) Bt protein exists as active toxin in the Bacillus.
b) The activated toxin enters the ovaries of the pest to sterilize it and thus prevent its multiplication.
c) The concerned Bacillus has antitoxins.
d) The inactive protoxin gets converted into active form in the insect gut.
Answer: d

Question: Which one of the following statement(s) is/are correct about Genetic Engineering Approval committee (GEAC)?
a) It will make decision regarding the validity of GM research.
b) It will make the safety of introducing GM–organism for public services.
c) Its genetic modification of organism can have unpredictable results when such organisms are introduced into the ecosystem. Therefore, the Indian government has set up organisation such as GEAC.
d) All of the above
Answer: d

Question: Which of the following is a correct statement?
a) “Bt” in Bt-cotton indicates that it is genetically modified organism produced through biotechnology.
b) Somatic hybridization involves fusion of two complete plant cells carrying desired genes.
c) The anticoagulant hirudin is being produced from transgenic Brassica napus seeds.
d) “Flavr Savr” variety of tomato has enriched the production of ethylene which improves its taste.
Answer: c

Question: Which of the following statements is correct?
a) The current interest in the manipulation of microbes, plants and animal has raised serious ethical issues.
b) One possible risk of genetic engineering is the accidental production of dangerously resistant microorganisms.
c) Although risks are possible, genetic engineering appears to offer more of contribution to human welfare than threats.
d) All of the above
Answer: d

Question: Which of the following statements (i–v) is/are incorrect?
(i) Recombinant DNA technology is used to improve crop plants by increasing their productivity, by making them more nutritious and by developing disease resistant.
(ii) Bt cotton is resistant to bollworm infestation.
(iii) Bacillus thuringiensis can form cry protein during any phase of their growth.
(iv) Bacillus thuringiensis is not harmed by self cry protein because of its occurrence as protoxin (inactive).
(v) Protoxin cry protein is changed into active cry protein in the stomach of insects due to alkaline pH in stomach.
a) Only (iii)
b) (i) and (iv)
c) All of these
d) None of these
Answer: c

Question: Read the following statements and choose the correct statements.
(i) Gene therapy has been tested on a large number of patients with a wider variety of inherited genetic disorders, and in numerous cases it has produced a complete cure.
(ii) Genetic engineering has been used to produce insulin for curing diabetes.
(iii) DNA hybridization is the base pairing of DNA from two different sources.
(iv) Genetic engineering is a technique of plant breeding.
a) (i) and (ii)
b) (ii) and (iii)
c) (i), (ii) and (iii)
d) All of these
Answer: b

Question: Transgenic animals are produced
(i) to study how genes are regulated and how they affect normal functions of body and its development.
(ii) to study diseases.
(iii) to obtain useful biological products.
(iv) to test vaccine safety and chemical safety.
a) (i), (ii), (iii) and (iv)
b) (i) and (iv)
c) (ii) and (iv)
d) Only (i)
Answer: a

Question: The given statements are the steps in one type of gene therapy. The correct sequence is:
(i) Inject engineered cells into patient’s bone marrow.
(ii) Viral DNA carrying the normal allele inserts into chromosome.
(iii) Let retrovirus infect bone marrow cells removed from patient and cultured.
(iv) Insert RNA version of normal allele into retrovirus.
a) (i), (ii), (iii) and (iv)
b) (iv), (iii), (ii) and (i)
c) (i), (ii), (iv) and (iii)
d) (iv), (iii), (i) and (ii)
Answer: b

Assertion/Reason Type Questions

In the following questions, a statement of Assertion is followed by a statement of Reason.
(a) If both Assertion and Reason are true and the Reason is the correct explanation of the Assertion.
(b) If both Assertion and Reason are true but the Reason is not the correct explanation of the Assertion.
(c) If Assertion is true but Reason is false.
(d) If both Assertion and Reason are false.

Question: Assertion : An application of tissue culture is the production of transgenic plants.
Reason : A transgenic organism is one that contains and expresses a transgene.
Answer: b

Question: Assertion : ELISA is based on the principle of antigen antibody interaction.
Reason : Pathogen infection is usually detected by presence of antigens or detection of antibodies synthesized against the pathogen.
Answer: a

Matching Type Questions

Question: Match column-I with column-II and identify the correct option.

(a) A – II; B – I; C – IV; D – III
(b) A – III; B – I; C – II; D – IV
(c) A – II; B – III; C – I; D – IV
(d) A – IV; B – I; C – II; D – III
Answer: b

Question: Match the following and choose the correct combination from the options given below :

(a) A – II; B – I; C – (IV); D – III
(b) A – II; B – I; C – (III); D – IV
(c) A – II; B – III; C – I; D – IV
(d) A – IV; B – I; C – II; D – III
Answer: c

Question: Match column-I with column-II and choose the correct option.

(a) A – II; B – I; C – IV; D – III
(b) A – II; B – I; C – III; D – IV
(c) A – II; B – III; C – I; D – IV
(d) A – IV; B – I; C – II; D – III
Answer: d