CBSE Class 12 Biology Biotechnology and Its Applications MCQs Set G

Question: The site of production of ADA in the body is
a) bone marrow
b) lymphocytes
c) blood plasma
d) monocytes
Answer: b

Question: Biopiracy is related to
a) bioresearches
b) traditional knowledge
c) biomolecules and genes discovered
d) all of the above
Answer: d

Question: Which one of the following techniques made it possible to genetically engineer living organisms?
a) Recombinant DNA techniques
b) X-ray diffraction
c) Heavier isotope labelling
d) Hybridization
Answer: a

Question: Cry-genes have been introduced in
a) cotton and corn
b) rice
c) potato and soyabean
d) all of the above
Answer: d

Question: Genes of interest can be selected from a genomic library by using
a) restriction enzymes
b) cloning vectors
c) DNA probes
d) gene targets
Answer: c

Question: The genetically-modified (GM) brinjal in India has been developed for
a) insect-resistance
b) enhancing shelf life
c) enhancing mineral content
d) drought-resistance
Answer: a

Question: C-peptide of human insulin is
a) a part of mature insulin molecule
b) responsible for formation of disulphide bridges
c) removed during maturation of pro-insulin to insulin
d) responsible for its biological activity
Answer: c

Question: Bacillus thuringiensis (Bt) strains have been used for designing novel
a) bio-fertilizers
b) bio-metallurgical techniques
c) bio-mineralization processes
d) bio-insecticidal plants
Answer: d

Question: Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produced (in the host cells)
a) both sense and anti-sense RNA
b) a particular hormone
c) an antifeedant
d) a toxic protein
Answer: a

Question: A transgenic food crop which may help in solving the problem of night blindness in developing countries is
a) golden rice
b) Bt soyabean
c) flavr-savr tomato
d) starlink maize
Answer: a

Question: E. coli are used in production of
a) rifampicin
b) LH
c) ecdysone
d) interferon
Answer: d

Question: Which of the following statement is correct about Bt toxin?
a) Bt protein exists as active toxin in the Bacillus
b) The activated toxin enters the ovaries of the pest to sterilize it and thus prevent its multiplication
c) The concerned Bacillus has antitoxins
d) The inactive protoxin gets converted into active form in the insect gut
Answer: d

Question: Which variety of rice was patented by a U.S. company even though the highest number of varieties of this rice is found in India?
a) Sharbati Sonara
b) Co-667
c) Basmati
d) Lerma Roja
Answer: c

Question: The genetic defect, adenosine deaminase (ADA) deficiency may be cured permanently by
a) administering adenosine deaminase through injection
b) bone marrow transplantation
c) enzyme replacement therapy
d) introducing isolated gene from marrow cells producing ADA into the cells at early embryonic stages
Answer: d

Question: Maximum number of existing transgenic animals is of
a) fish
b) mice
c) cow
d) pig
Answer: b

Question: The first clinical gene therapy was given in 1990 to a 4 years old girl with enzyme deficiency of
a) adenosine deaminase (ADA)
b) tyrosine oxidase
c) monamine oxidase
d) glutamate dehydrogenase
Answer: a

Question: Cry protein is obtained from
a) Bacillus thuringiensis
b) Bacillus subtilis
c) Clostridium welchii
d) E. coli
Answer: a

Question: Transgenic plants are the ones
a) generated by introducing foreign DNA into a cell and regenerating a plant from that cell
b) produced after protoplast fusion in artificial medium
c) grown in artificial medium after hybridization in the field
d) produced by a somatic embryo in artificial medium
Answer: a

Question: Which one of the following statement(s) is/are correct about Genetic Engineering Approval Committee (GEAC)?
a) It will make decision regarding the validity of GM research
b) It will make the safety of introducing GM-organism for public services
c) Its genetic modification of organism can have unpredictable results when such organisms are introduced into the ecosystem. Therefore, the Indian government has set up organisation such as GEAC
d) All of the above
Answer: d

Question: Which of the following Bt crops is being grown in India by the farmers?
a) Cotton
b) Brinjal
c) Soyabean
d) Maize
Answer: a

Question: Which one of the following statement is correct?
a) The proteins encoded by the genes cry I Ac and cry II Ab control cotton bollworms.
b) Protein encoded by cry I Ab controls corn borer.
c) Both (a) and (b)
d) Proteins encoded by cry I Ac and cry I Ab control flies.
Answer: c

Question: In some children, ADA deficiency can be cured by
a) bone marrow transplantation
b) enzyme replacement therapy
c) both (a) and (b)
d) none of the above
Answer: c

Question: DNA or RNA segment tagged with a radioactive molecule is called
a) vector
b) probe
c) clone
d) plasmid
Answer: b

Question: Which of the following statement(s) is/are correct?
a) The procedure for chemical safety testing/toxicity is the same as that used for testing toxicity of drugs.
b) Transgenic animals are more sensitive to toxic substances than non-transgenic animals.
c) Golden rice, a genetically engineered rice has high vitamin A (retinol) content.
d) All of the above
Answer: d

Question: GEAC stands for
a) Genome Engineering Action Committee
b) Ground Environment Action Committee
c) Genetic Engineering Approval Committee
d) Genetic and Environment Approval Committee
Answer: c

Question: The use of bioresources by multinational companies and other organizations without proper authorization from the countries and people concerned without compensatory payment is called
a) bioethics
b) biopiracy
c) bioterror
d) bioweapon
Answer: b

Question: Consumption of which one of the following foods can prevent the kind of blindness associated with vitamin A deficiency?
a) ‘Flavr Savr’ tomato
b) Canola
c) Golden rice
d) Bt-brinjal
Answer: c

Question: Transgenic animals have been successfully used for producing
a) transgenic mice for testing safety of polio vaccine before use in humans
b) transgenic models for studying new treatments for certain cardiac diseases
c) transgenic cow Rosie which produces high fat milk for making ghee
d) animals like bulls for farm work as they have super power
Answer: a

Question: Which step of Government of India has been taken to cater requirement of patent terms and other emergency provisions in this regard?
a) Biopiracy Act
b) Indian Patents Bill
c) RTI Act
d) Negotiable Instruments Act
Answer: b

Question: Find out the incorrect statement.
a) Human protein used to treat emphysema is α-1 antitrypsin.
b) Human insulin is being commercially produced from a transgenic species of Agrobacterium tumefaciens.
c) Rosie, the first transgenic cow, produced human protein enriched milk.
d) Cry I Ab endotoxin obtained from Bacillus thuringiensis is effective against corn borers.
Answer: b

Question: The protein α-1 antitrypsin is used to treat
a) cancer
b) rheumatoid arthritis
c) Alzheimer’s disease
d) emphysema
Answer: d

Question: The first human drug made using recombinant DNA technology was
a) glyphosatase
b) TPA
c) insulin
d) erythropoietin
Answer: c

Question: The transgenic animals are those which have
a) foreign DNA in some cells
b) foreign DNA in all of their cells
c) foreign RNA in all of their cells
d) both (a) and (c)
Answer: b

Question: RNA interference (RNAi) technique has been devised to protect plants from nematode. It is silenced by ______ produced by the host plant.
a) dsDNA
b) ssDNA
c) dsRNA
d) target proteins
Answer: c

Question: Which one of the following is a correct statement?
a) “Bt” in Bt-cotton indicates that it is a genetically modified organism produced through biotechnology.
b) Somatic hybridization involves fusion of two complete plant cells carrying desired genes.
c) The anticoagulant hirudin is being produced from transgenic Brassica napus seeds.
d) “Flavr Savr” variety of tomato has enriched the production of ethylene which improves its taste.
Answer: c

Question: Bt toxin is harmful to insects like
a) Lepidopterans (tobacco budworm, armyworms)
b) Coleopterans (beetles)
c) Dipterans (flies and mosquitoes)
d) all of the above
Answer: d

Question: How many varieties of rice have been estimated to be present in India?
a) 2,000
b) 20,000
c) 200,000
d) 2,000,000
Answer: c

Question: Select the correct statement.
a) Genetic engineering works only on animals and has not yet been successfully used on plants.
b) There are no risks associated with DNA technology.
c) The first step in PCR is heat which is used to separate both the strands of target DNA.
d) DNA from one organism will not bond to DNA from another animal.
Answer: c

Question: Which of the following statements is correct?
a) The current interest in the manipulation of microbes, plants and animals has raised serious ethical issues.
b) One possible risk of genetic engineering is the accidental production of dangerously resistant microorganisms.
c) Although risks are possible, genetic engineering appears to offer more contribution to human welfare than threats.
d) All of the above
Answer: d

Question: Silencing of mRNA has been used in producing transgenic plants resistant to
a) bollworms
b) nematodes
c) white rusts
d) bacterial blights
Answer: b

Question: Which of the following is true for Golden rice?
a) It has yellow grains, because of a gene introduced from a primitive variety of rice.
b) It is vitamin A enriched, with a gene from daffodil.
c) It is pest resistant, with a gene from Bacillus thuringiensis.
d) It is drought tolerant, developed using Agrobacterium vector.
Answer: b

Question: Which of these is used as vector in gene therapy for SCID?
a) Arbovirus
b) Rotavirus
c) Retrovirus
d) Parvovirus
Answer: c

Question: The 51 amino acids of insulin are arranged in
a) single polypeptide
b) two polypeptides, having 21 and 30 amino acids in chain A and B respectively
c) two polypeptides having 25 and 26 amino acids
d) two polypeptides having 18 and 33 amino acids
Answer: b