CBSE Class 12 Biology HOTs Biotechnology Principles and Processes

Please refer to CBSE Class 12 Biology HOTs Biotechnology Principles and Processes. Download HOTS questions and answers for Class 12 Biology. Read CBSE Class 12 Biology HOTs for Chapter 11 Biotechnology Principles and Processes below and download in pdf. High Order Thinking Skills questions come in exams for Biology in Class 12 and if prepared properly can help you to score more marks. You can refer to more chapter wise Class 12 Biology HOTS Questions with solutions and also get latest topic wise important study material as per NCERT book for Class 12 Biology and all other subjects for free on Studiestoday designed as per latest CBSE, NCERT and KVS syllabus and pattern for Class 12

Chapter 11 Biotechnology Principles and Processes Class 12 Biology HOTS

Class 12 Biology students should refer to the following high order thinking skills questions with answers for Chapter 11 Biotechnology Principles and Processes in Class 12. These HOTS questions with answers for Class 12 Biology will come in exams and help you to score good marks

HOTS Questions Chapter 11 Biotechnology Principles and Processes Class 12 Biology with Answers

Question. Why foreign DNA cannot pass through cell membrane?
(a) DNA is hydrophobic
(b) DNA is hydrophilic
(c) DNA is rich in proteins
(d) DNA is heavy
Answer : B

 

Question. Which of the following has popularised the PCR (polymerase chain reactions)?
(A) Easy availability of DNA template
(B) Availability of synthetic primers
(C) Availability of cheap deoxyribonucleotides
(D) Availability of ‘Thermostable’ DNA polymerase.
Answer : D

Question. Which of the following steps are catalysed by Taq polymerase in a PCR reaction?
(A) Denaturation of template DNA.
(B) Annealing of primers to template DNA.
(C) Extension of primer end on the template DNA.
(D) All of the above. 
Answer : C

Question. The role of DNA ligase in the construction of a recombinant DNA molecule is
(A) Formation of phosphodiester bond between two DNA fragments.
(B) Formation of hydrogen bonds between sticky ends of DNA fragments.
(C) Ligation of all purine and pyrimidine bases.
(D) None of the above 
Answer : A

Question. Consider the following statements and select the correct option.
(a) A soil inhabiting plant bacterium, Agrobacterium tumefaciens, a pathogen of several dicot plants is able to transfer a piece of DNA known as T-DNA
(b) The T-DNA causes tumours
(c) Tumour formation is induced by Ti plasmid
(d) All of the above
Answer : D

Question. Which of the following statement is incorrect?
(a) Each restriction endonuclease recognises a specific palindromic nucleotide sequence
(b) Specific base sequence is known as recognition sequence
(c) Restriction enzymes cannot cut DNA
(d) Restriction enzymes belong to enzymes called nucleases
Answer : C

Question. Which of the following statement is incorrect?
(a) EcoRI cuts the DNA between bases G and A
(b) Making multiple identical copies of any template DNA is called cloning
(c) pBR322 is a natural plasmid
(d) Agrobacterium tumefaciens is a natural genetic engineer
Answer : C

Question. Which of the following statements does not hold true for restriction enzyme?
(A) It recognises a palindromic nucleotide seq-uence.
(B) It is an endonuclease.
(C) It is isolated from viruses.
(D) It produces the same kind of sticky ends in different DNA Molecules. 
Answer : C

Question. Which of the following should be choosen for best yield if one were to produce a recombinant protein in large amounts?
(A) Laboratory flask of largest capacity.
(B) A stirred-tank bioreactor without in-lets and outlets.
(C) A continuous culture system.
(D) Any of the above.
Answer : C

Question. Choose the incorrect statement.
(a) Ori also controls the copy numbers of the linked DNA
(b) If a foreign DNA ligates at the Bam HI site of tetracycline resistance gene in the vector pBR322, the recombinant plasmid loses the tetracycline resistance due to insertion of foreign DNA
(c) Copy number refers to the number of copies of plasmid present in a cell
(d) Copy number of plasmid varies from 50-100 per cell
Answer : D

Question. Which of the statements given is incorrect?
(a) In microinjection method, foreign DNA is directly injected into the nucleus of animal cell by using microneedles
(b) Microinjection method is used in oocytes, eggs and embryo
(c) Electroporation is the formation of temporary pores in the plasma membrane of host cell by using lysozyme or calcium chloride
(d) In chemical mediated gene transfer method, certain chemicals such as Ca phosphate help foreign DNA to enter the host cell
Answer : C

Question. Consider the following statements.
I. RecombinantDNAtechnology popularly known as genetic engineering is a stream of biotechnology which deals with the manipulation of genetic material by manin vitro.
II. pBR322 is the first artificial cloning vector developed in 1977 by Boliver and Rodriquez from E. coli plasmid.
III. Restriction enzymes belong to a class of enzymes called nucleases.
Which of the statements given above are correct?
(a) I and II
(b) I and III
(c) II and III
(d) I, II and III
Answer : D

Question. Which of the given statement is correct in the context of observing DNA separated by agarose gel electrophoresis?
(A) DNA can be seen in visible light.
(B) DNA can be seen without staining in visible light.
(C) Ethidium bromide stained DNA can be seen in visible light.
(D) Ethidium bromide stained DNA can be seen under exposure to UV light. 
Answer : D

Question. Who among the following was awarded the Nobel Prize for the development of PCR technique?
(A) Herbert Boyer
(B) Hargovind Khurana
(C) Kary Mullis
(D) Arthur Kornberg
Answer : C

Question. Given below are four statements pertaining to separation of DNA fragments using gel electrophoresis. Identify the incorrect statements.
I. DNAis negatively charged molecule and so it is loaded on gel towards the anode terminal.
II. DNAfragments travel along the surface of the gel whose concentration does not affect movement of DNA.
III. Smaller the size ofDNAfragment larger is the distance it travels through it.
IV. PureDNAcan be visualised directly by exposing UV-radiation.
Select the correct option from the following.

(a) I, III and IV
(b) I, II and III
(c) II, III and IV
(d) I, II and IV
Answer : D

Question. The treatment of host cell with divalent cation leads to the
(a) change in permeability of DNA
(b) increased efficiency with which DNA enters the bacterium
(c) decreased efficiency with which DNA enters the bacterium
(d) change in permeability of host
Answer : B

Question. Which vector can clone only a small fragment of DNA?
(a) Bacterial artificial chromosome
(b) Yeast artificial chromosome
(c) Plasmid
(d) Cosmid
Answer : C

Question. The DNA used as a carrier for transferring a fragment of foreign DNA into a suitable host is called 
(a) cloning vector
(b) vehicle DNA
(c) gene carrier
(d) All of these
Answer : D

Question. Which of the following is a plasmid vector?
(a) pBR322
(b) Bam II
(c) SaI I
(d) Eco RI
Answer : A

Question. The method which is used to introduce recombinant DNA into animal cell?
(a) Gene gun method
(b) Changing permeability of host
(c) Biolistic method
(d) Microinjection
Answer : D

Question. Which of the following methods(s) is used to introduce foreign DNA into plant host cells?
(a) Gene gun method
(b) Gel electrophoresis
(c) Elution
(d) Extension
Answer : A

Question. For transformation, microparticles coated with DNA to be bombarded with gene gun are made up of
(a) silver or platinum
(b) platinum or zinc
(c) silicon or platinum
(d) gold or tungsten
Answer : D

Question. DNA transfer with high velocity micro particles is present in
(a) biolistics
(b) hybridisation
(c) tissue culture
(d) vegetative propagation
Answer : A

Question. The different steps involved in the process of recombinant DNA technology are given below randomly? Arrange these in correct order.
I. Extraction of the desired gene product.
II. Amplification of the gene of interest.
III. Isolation of a desiredDNAfragment.
IV. Ligation of theDNAfragment into a vector.
V. Insertion of recombinantDNAinto the host.
Correct order is
(a) I, II, III, IV and V
(b) III, II, IV, V and I
(c) II, IV, V, III and I
(d) I, IV, V, III and II
Answer : B

Question. In bacterial cells, the membrane is digested with the help of enzyme
(a) cellulase
(b) lysozyme
(c) chitinase
(d) lipase
Answer : B

Question. Primers are
(a) small chemically synthesised oligonucleotides of about 10-18 nucleotides that are complementary to the region of template DNA
(b) chemically synthesised oligonucleotides of about 10-18 nucleotides that are not complementary to the region of template DNA
(c) the double-stranded DNA that need to be amplified
(d) specific sequences present on recombinant DNA
Answer : A

Question. The Taq polymerase enzyme is obtained from
(a) Thiobacillus ferroxidans
(b) Bacillus subtilis
(c) Pseudomonas subtilis
(d) Thermus aquaticus
Answer : D

Question. A single PCR amplification cycle involves
(a) denaturation
(b) extension
(c) annealing
(d) All of these
Answer : D

Question. In gel electrophoresis, restriction enzyme digested DNA is loaded in wells near
(a) anode
(b) cathode
(c) centre of gel
(d) any where in the gel
Answer : B

Question. Stirred-tank bioreactors are advantageous over shake flasks because they
(a) provide high temperature and pH
(b) provide better aeration and mixing properties
(c) do not allow the entry of CO2
(d) are easy to operate
Answer : B

Question. The components of a bioreactor are
I. an agitator system.
II. an oxygen delivery system.
III. foam control system.
IV. temperature control system.
V. pH control system.
VI. sampling ports towithdrawcultures periodically.
Choose the correct option.
(a) I, II, III, IV and V
(b) II, IV, V and VI
(c) I, II, III, IV and VI
(d) All of these
Answer : D

Question. The process of separation and purification of expressed protein before marketing is called
(a) upstream processing 
(b) downstream processing
(c) bioprocessing
(d) post-production processing
Answer : B

Question. Which of the following is not a component of downstream processing?
(a) Separation
(b) Purification
(c) Preservation
(d) Expression
Answer : D

I. Assertion and Reason
In each of the following questions, a statement of Assertion (A) is given followed by corresponding statement of Reason (R). Of the statements, mark the correct answer as
(a) If both A and R are true and R is the correct explanation of A
(b) If both A and R are true, but R is not the correct explanation of A
(c) If A is true, but R is false
(d) If A is false, but R is true

Question. Assertion (A) Biotechnology deals with techniques that use living organism to produce products useful for humans.
Reason (R) It uses only a unicellular organism.
Answer : C

Question. Assertion (A) Maintenance of sterile environment is essential for manufacture of biotechnological products.
Reason (R) This is to enable growth of desired prokaryotic or eukaryotic cells.
Answer : A

Question. Assertion (A) Origin of replication is an essential part of a vector.
Reason (R) Ori is responsible for initiating replication.
Answer : A

Question. Assertion (A) Foreign DNA and vector DNA cut with the help of ligase.
Reason (R) Ligase acts by forming phosphodiester bonds.
Answer : D

Question. Assertion (A) In gel electrophoresis, DNA fragments are separated.
Reason (R) DNA is negatively charged, so it moves towards anode under electric field.
Answer : A

Question. Assertion (A) Restriction endonucleases are also called ‘molecular scissors’.
Reason (R) When fragments generated by restriction endonucleases are mixed, they join together due to their sticky ends.
Answer : B

Question. Assertion (A) All endonucleases cut DNA at specific sites.
Reason (R) Endonucleases were discovered from viruses.
Answer : C

Question. Assertion (A) The tumour inducing plasmid (Ti plasmid) of Agrobacterium tumefaciens acts as a cloning vector in recombinant DNA technology.
Reason (R) The Ti plasmid which is used in the mechanisms of delivering genes to a cell remains pathogenic.
Answer : C

Question. Assertion (A) Use of chitinase enzyme is necessary for isolation of DNA from fungal cells.
Reason (R) Fungal cell wall is made up of chitin and chitinase is able to digest it.
Answer : A

Statement Based Questions

Question. Which one is a true statement regarding DNA polymerase used in PCR?
(a) It is used to ligate introduced DNA in recipient cells
(b) It serves as a selectable marker
(c) It is isolated from a virus
(d) It remains active at high temperature
Answer : D

Question. Following statements describe the characteristics of the enzyme restriction endonulease. Identify the incorrect statement. NEET 2019
(a) The enzyme binds DNA at specific sites and cuts only one of the two strands
(b) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand
(c) The enzyme recognises a specific palindromic nucleotide sequence in the DNA
(d) The enzyme cuts DNA molecules at identified position within the DNA
Answer : A

Question. Which of the following statement is incorrect?
(a) Nucleic acid is fragmented by nucleases
(b) Construction of recombinant DNA involves cleaning DNA segments with endonuclease and rejoining with ligase
(c) Genetic engineering is making artificial limbs and diagnostic instruments
(d) Ti plasmid transforms cells of plants
Answer : C

Question. Which of the following statement is incorrect?
(a) DNA being a hydrophilic molecule cannot pass through cell membranes
(b) Agrobacterium tumefaciens delivers a piece of DNA known as ‘Z-DNA’ which transforms normal plant cells into tumour cells and directs these tumour cells to produce chemicals against pathogens
(c) Retrovirus, adenovirus, papillomavirus are also now used as cloning vectors in animal because of their ability to transform normal cells into cancerous cell
(d) In genetic engineering, DNA from different sources are cut with the same restriction enzymes so that both DNA fragments have same kind of sticky ends
Answer : B

 
Q.1 
CBSE_Class_12_Biology_Biotechnology_&_its_Application_1
 
Ans.1
CBSE_Class_12_Biology_Biotechnology_&_its_Application_2
Q.2
CBSE_Class_12_Biology_Biotechnology_&_its_Application_3
 
Answer the questions from the figure given
i) W rite the name of the enzyme required for PCR.
ii) From which organism it is obtained?
Iii) What is the significance of this enzyme ?
 
Ans.i)Taq polymrrase
     ii)Thermus aquaticus
     iii) Thermostable
 
Q3. Match the following
                  A                                                                         B
          i) Bacteria                                                             i) Celluase
ii) Chitinase                                           ii)Microparticles of gold or tungsten coated with DNA
iii) Plant cell                                                                  iii) Microinjection
iv) Recombinant DNA injected
to nucleus of an animal cell                                             iv) Lysozyme
v) Biolistic /Gene gun                                                       v) Fungus
 
Ans  Match the following
                  A                                                                         B
Bacteria --- ----------------------                                         Lysozyme
Chitinase ---------------------                                                Fungus
Biolistic /Gene gun -----------                        Microparticles of gold or tungsten coated with DNA
Plant cell ---------------------                            Celluase Recombinant DNA injected to
nucleus of an animal cell ------ ------                                  Microinjection
 
Q4. Write the pallinodromic sequence of the following     5'-------GAATTC--------3'
 
Ans.4 3'------CTTAAG-------5'
 
Q5
CBSE_Class_12_Biology_Biotechnology_&_its_Application_4
i)What dose the fig shows?
ii)What is the matrix used for the above process?
Iii)Name the chemical compound used for staining?
iv)Name the process for the separation of DNA from the agarose gel.
 
Ans. i) Agarose gel electrophoresis ii) Agarose
iii) Ethidium bromide
iv) Elution

Q.1. Agrobacterium tumefaciens is called natural genetic engineer. It infects only dicot plants. Unfortunately, many important crop plants, including corn, rice, and wheat, are monocots and thus could not be easily transfected using this bacterium. Scientists discovered that by using certain processes, naked DNA molecules can be introduced into plant cell types that are not susceptible to A. tumefaciens transfection. Explain any two.

 
Ans1. a. Micro-injection where r-DNA is directly injected into the nucleus of the host cell.
b. Cells are bombarded with high velocity micro-particles of gold or tungsten coated with the desired gene with its operating system ( Biolistics /gene gun ).
 
Q.2 Each restriction enzyme cuts DNA at a specific base sequence. For example, EcoRI always cuts DNA at GAATTC
3’—GTAAGAATTCTTTAGAATTCCGCCATTATCGAATTCAGGATCTTAC—5’
5’—CATTCTTAAGAAATCTTAAGGCGGTAATAGCTTAAGTCCTAGAATG—3’
a. How many times EcoRI cuts this long strand?
b. How many small DNA pieces are formed from this long strand?
c. These fragments show overhangs of single stranded DNA. What are they known as? Why are they called so?
d. Which enzyme is used to seal these fragments together?
 
Ans2. a. Three cuts.
b. Four strands.
c. Sticky ends, since they have complementary bases, they can rejoin fragments with complementary sticky ends to form rDNA (gene from vector and the gene to be cloned is inserted)
d. DNA ligase
 
Q.3. Any DNA flanked at its two borders by T-DNA can be integrated into the plant genome. Name the vector used for this plant gene transfer?
 
Ans.3. Tumor inducing plasmids (Ti Plasmids)
 
Q.4. A dicot leaf is punched and is incubated in a suspension of soil bacteria. The cells on the edge of the punch are transformed and developed into new plants through tissue culture.
a. Name the technology.
b. Name the soil bacteria responsible for this transformation.
 
Ans.4. a. DNA recombinant technology
b. Agro bacterium termifaciens- a natural genetic engineer.
 
Q.5. Dicot plants do show tumorous growth (crown gall disease) on the stem.
a. Name the microorganism responsible for this growth?
b. What causes the production of tumors?
 
Ans5. a. Agro bacterium termifaciens
    b. The transfer of bacterial DNA to the plant which integrates into the plant genome and produces hormones for the over growth
 
Q.6. In Gel electrophoresis, the DNA fragments are placed in wells on a sheet of gelatin and an electric current is applied to the sheet. Based on DNA biochemistry in which side are the wells located? In which direction and why do the fragments migrate?
 
Ans 6. DNA is loaded on the cathode side of the gel. Since DNA is negatively charged the fragments move towards positive pole (anode) when the electric field is applied.
 
Q.7. Agrobacterium tumifaciens is a pathogen of several dicot plants. But for biotechnologists it is a boon. Justify?
 
Ans7. It is considered as a natural genetic engineer. The Ti-plasmid of the bacterium is modified and used as a cloning vector to deliver the genes of our interest into a variety of plants.
 
Q.8. Suppose you want to make a billion copies of a small region of the chromosome in vitro, which biotechnological mechanism will you prefer? Name the three main steps involved?
 
8. Polymerase chain reaction to amplify gene of interest.
(i) Denaturation
(ii) Annealing
(iii) Extension
 
Q.9. A foreign DNA fragment is introduced into the plasmid at the ‘Bam H-1’ site of the tetracycline resistant gene in the vector ‘pBR 322’.
a) How does it affect the recombinant plasmid?
b) What is this mechanism known as?
c) Mention its advantages?
 
Ans9. a. Due to insert the recombinant plasmids loose the tetracycline resistance.
    b. Insertional inactivation.
    c. To select recombinants from non-recombinants.
 
Q.10. In nature A. tumifaciens which is called a natural genetic engineer, infects only dicots. Unfortunately many important crop plants are monocots and thus could not be easily transfacted. Scientists have discovered certain processes to introduce naked DNA molecules into the plant tissue that are not susceptible to A. tumefactions ? Explain any two.
 
Ans10 For explanation refer text book.
      a. Micro-injection
      b. Biolistics
 
Q.11. In making bacteria as a competent host for transformation with r-DNA, it is treated with a specific concentration of divalent cation like calcium. What is the role of calcium?
 
Ans11. Creates pores in its cell wall
 
Q.12. After completion of the biosynthetic stage the products has to be subjected through a series of processes before it is ready for marketing.
a) List these processes?
b) What are they collectively known as?
c) List any other measures to be taken care of?
 
Ans12.a. Separation, purification
    b. Downstream processing
    c. Quality control testing
 
Q.13. Traditional hybridization procedures used in plant and animal breeding very often leads to inclusion and multiplication of undesirable genes along with the desirable ones. What are the steps involved in genetic engineering to over come this limitation?
 
Ans13 (i) Gene cloning
(ii) Gene transfer
 
Q.14. Selection of recombinants due to inactivation of antibiotics is a cumbersome procedure. Why? Explain the alternative method to overcome this difficulty?
 
Ans14(i) It requires simultaneous planting on two plants having different antibiotics.
(ii) Development & use of selectable marker which differentiate recombinats from nonrecombinats on the basis of there ability to produce colour in the process of a chromogenic substance.
 
15. Following figures show a biotechnological Procedure which requires template DNA strand, primers, DNA polymerase, and nucleotides.
a..Name the procedure.
b.List and explain the steps shown in figures 2 - 5
CBSE_Class_12_Biology_Principles_&_Processes_1
Ans15(i) Polymerase chain reaction to amplify gene of interest
(ii) fig. 2 – Denaturation-DNA is heated appox..95oC to separate the two stands of the double helix.
(iii) Fig.3. Attachment of the primers & then polymerize after cooling annealing.
(iv) Fig .3 Extension & amplification

 

Q.1Restriction Enzymes are called “Molecular scissors”. In what context they are referred so?
 
Ans 1.Theycut DNA molecules at specific sites and then fragment
 
Q.2 In what way the following two plasmids differ? 1
CBSE_Class_12_Biology_Principles_And_Processes_1
Ans2 a- non recombinat b. recembinqat
 
Q.3. What is the source of thermostable DNA polymerase and Name that polymerase. Why thermostable DNA polymerase is essential in PCR? 1
 
Ans3 Bacteria – Thermus aquaticus: Taq polymerase: During Denaturation in PCR high temperature is required and thermostable DNA polymerase remains stable in that high temperature.
 
Q.4 “The prophase I of meiosis plays a vital role in r-DNA formation” Justify the statement.1

Ans4 crossing over occurs in this phase helps to produce recombinants.
 
Q.5 Eukaryotes do not have restriction endonuclease, then how they manage with normal endonuclease enzyme?1
 
Ans5 I n Eukaryotes, normal endonuclease attach with special proteins, like initiator, terminator, Snrbs etc. to perform their functions.
 
Q.6What special feature do prokaryotes have to defend themselves from bacteriophages?1
 
Ans6 .Restriction Endonuclease enzyme.
 
Q.7_____= Denaturation +______+Extenton.1
 
Ans7 .PCR, Annealing.
 
Q.8.Complete the following palindrome sequence and name the restriction endonuclese that recognises this.1
CBSE_Class_12_Biology_Principles_And_Processes_2
Ans.8
CBSE_Class_12_Biology_Principles_And_Processes_3
2 MARKS
 
Q.1) Is advisable to use different restriction endonucleases to cut the vector DNA and source DNA? Why? 2
 
Ans1) No, when cut with the same restriction enzyme, only the resultant DNA fragments have the same sticky ends and these can be joined together using DNA ligases.
 
Q 2) What will happen if more than one recognition sites are present in a vector?2
 
Ans2) Presence of more than one recognition sites within the vector will generate several fragments, which will complicate gene cloning.
 
1. These are enzymes called restriction enzyme found in bacteria and are used to cut pieces of DNA
 
2. These are organisms that are used to transfer a gene of interest to a host. Eg: plasmid, virus, and bacteria.
 
Q.3 Uncontrolled recombinant DNA technology experiments is dangerous to mankind. Comment on it.
 
Ans 3. The palindrome in DNA is a sequence of base pains that read same on the two strands when orientation of reading is kept same. E.g.
 
5’ – GAATTC – 3’
3 – CTTAAG – 5
 
Q.4
 
  CBSE_Class_12_Biology_Principles_And_Processes_4.
Ans 4
CBSE_Class_12_Biology_Principles_And_Processes_5
Q.5. (a) Which is the most commonly used matrix in gel electrophoresis ?
       (b) What is the source of it?
 
Ans.5(a) Agrose.
         (b) Sea weed-Gelidium sp.
 
Q.6 Find the ‘odd one out and write why that is ‘odd’
      (a) Sal I, Pst I, Cla I, BamH I, pBR 322
      (b) Bacteria, Virus, Gene-gun, Fungi
 
Ans.6 (a) pbr 322, it is a plasmid-vector; others restriction enzymes in the plasmid.
          (b) gene-gun- it is a device used for direct introduction of a gene in a host cell.
 
Q.7 Detect the mismatch from the following and replace the wrong match with a right one
(a) ECOR I –Bacteria
(b) Ethidium Bromidqe- Gel electrophoresis
(c) Lysozyme- Fungi
(d) Palindrome sequence-Restriction enzyme
Resistant gene.
(b)Cellulose,__________,__________,fungus
 
Ans.7 Lysosome -fungi,.(lysozyme is the enzyme to related to bacteria and not fungi)
 
Q.8.Write the use of the following in Biotechnology.
 
       (a) Chilled ethanol       
           
       (b) Microinjection
 
       (c) Bioreactor
 
       (d) plasmid
 
Ans.8. (a) added to precipitate the purified DNA to isolate it.
 
         (b) microinjection- to inject the foreign gene into a host cell, directly.
 
         (c) set up to culture large volumes of transgenic bacteria to get large quantities of the product protein.
 
         (d) plasmid- the vector to transform a foreign gene.
 
Q.9. Is there any difference between recombinant DNA and recombinant protein? Support your answer.
 
Ans.9. Yes, r-DNA = plasmid(vector) + foreign DNA
          r-protein is the product of transgenic gene in the host body /cell.
 
3 MARKS
Q.1. Give the correct term for the following; -
 
(a) Replacing a defective mutant allele with a normal functional allele / gene.
 
(b) Increasing the copies (content) of a gene
 
(c) Preventing m-RNA translation
 
(d) Specific pattern of base pairs that are recognized by restriction enzymes.
 
(e) Mobile genetic element
 
(f) One DNA copies itself and produces two
 
Ans.1.(a) gene therapy
 
        (b) amplification
 
        (c) RNA interference
 
        (d) palindronic sequence
 
        (e)transposones
 
        (f) DNA replication
 
Q.2. Name the enzyme involved in the following process:
 
(a) Repeated amplification of DNA fragments.
 
(b) Formation of short piece of RNA strand for annealing.
 
(c) Breaking of bacterial cell to release DNA and other macromolecules.
 
(d) Cutting and rejoining DNA fragments.
 
(e) Formation of m-RNA.
 
Ans.2. (a) tag polymerase (DNA polymerase )
 
    (b) primase
 
    (c) lysozyme
 
   (d) topoisomerse
 
   (e)RNA- polymersease
 
   (f) DNA ligase

3 (a) Linking a foreign DNA with a plasmid(vector)
(b) DNA formed by a RNA by Reverse Transciption.
(c) ds RNA- complementary RNA
4.E.coli cloning vector
(a) ori
(b) rop
(c) ECOR I
(d) ampR
(e) tetR

5MARKS
.1. (a) “Insertional inactivation”
In this recombinants & non recombinants are differentiated on the basis of the ability to produce colour in the presence of a chromosomic substrate – In this, a rDNA is inserted
in an enzyme – α galactosidase – leads to inactivation of the enzyme which does not produce colour due to insertion.
(b)
(i) Host cells are incubated with rDNA on ice.
(ii) Followed by placing them briefly at 410C heat.
(iii) Then transforming them back on ice.
This enables the host cells (bacteria) to take up the rDNA.

Chapter 01 Reproduction in Organisms
CBSE Class 12 Biology HOTs Reproduction In Organisms
Chapter 02 Sexual Reproduction In Flowering Plants
CBSE Class 12 Biology HOTs Sexual Reproduction In Flowering Plants
Chapter 03 Human Reproduction
CBSE Class 12 Biology HOTs Human Reproduction
Chapter 04 Reproductive Health
CBSE Class 12 Biology HOTs Reproductive Health
Chapter 05 Principles of Inheritance and Variation
CBSE Class 12 Biology HOTs Principles Of Inheritance And Variation
Chapter 06 Molecular Basis of Inheritance
CBSE Class 12 Biology HOTs Molecular Basis of Inheritance
Chapter 08 Human Health and Disease
CBSE Class 12 Biology HOTs Human Health and Disease
Chapter 09 Strategies for Enhancement In Food Production
CBSE Class 12 Biology HOTs Strategies For Enhancement In food Production
Chapter 10 Microbes In Human Welfare
CBSE Class 12 Biology HOTs Microbes In Human Welfare
Chapter 11 Biotechnology Principles and Processes
CBSE Class 12 Biology HOTs Biotechnology Principles and Processes
Chapter 12 Biotechnology and Its Application
CBSE Class 12 Biology HOTs Biotechnology And Its Applications
Chapter 13 Organisms and Populations
CBSE Class 12 Biology HOTs Organism And Population
Chapter 15 Biodiversity and Conservation
CBSE Class 12 Biology HOTs Biodiversity And Conservation
Chapter 16 Environmental Issues
CBSE Class 12 Biology HOTs Environmental Issues

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CBSE Class 12 Biology Chapter 11 Biotechnology Principles and Processes HOTS

We hope students liked the above HOTS for Chapter 11 Biotechnology Principles and Processes designed as per the latest syllabus for Class 12 Biology released by CBSE. Students of Class 12 should download the High Order Thinking Skills Questions and Answers in Pdf format and practice the questions and solutions given in above Class 12 Biology  HOTS Questions on daily basis. All latest HOTS with answers have been developed for Biology by referring to the most important and regularly asked topics that the students should learn and practice to get better score in school tests and examinations. Studiestoday is the best portal for Class 12 students to get all latest study material free of cost.

HOTS for Biology CBSE Class 12 Chapter 11 Biotechnology Principles and Processes

Expert teachers of studiestoday have referred to NCERT book for Class 12 Biology to develop the Biology Class 12 HOTS. If you download HOTS with answers for the above chapter daily, you will get higher and better marks in Class 12 test and exams in the current year as you will be able to have stronger understanding of all concepts. Daily High Order Thinking Skills questions practice of Biology and its study material will help students to have stronger understanding of all concepts and also make them expert on all critical topics. You can easily download and save all HOTS for Class 12 Biology also from www.studiestoday.com without paying anything in Pdf format. After solving the questions given in the HOTS which have been developed as per latest course books also refer to the NCERT solutions for Class 12 Biology designed by our teachers

Chapter 11 Biotechnology Principles and Processes HOTS Biology CBSE Class 12

All HOTS given above for Class 12 Biology have been made as per the latest syllabus and books issued for the current academic year. The students of Class 12 can refer to the answers which have been also provided by our teachers for all HOTS of Biology so that you are able to solve the questions and then compare your answers with the solutions provided by us. We have also provided lot of MCQ questions for Class 12 Biology in the HOTS so that you can solve questions relating to all topics given in each chapter. All study material for Class 12 Biology students have been given on studiestoday.

Chapter 11 Biotechnology Principles and Processes CBSE Class 12 HOTS Biology

Regular HOTS practice helps to gain more practice in solving questions to obtain a more comprehensive understanding of Chapter 11 Biotechnology Principles and Processes concepts. HOTS play an important role in developing an understanding of Chapter 11 Biotechnology Principles and Processes in CBSE Class 12. Students can download and save or print all the HOTS, printable assignments, and practice sheets of the above chapter in Class 12 Biology in Pdf format from studiestoday. You can print or read them online on your computer or mobile or any other device. After solving these you should also refer to Class 12 Biology MCQ Test for the same chapter

CBSE HOTS Biology Class 12 Chapter 11 Biotechnology Principles and Processes

CBSE Class 12 Biology best textbooks have been used for writing the problems given in the above HOTS. If you have tests coming up then you should revise all concepts relating to Chapter 11 Biotechnology Principles and Processes and then take out print of the above HOTS and attempt all problems. We have also provided a lot of other HOTS for Class 12 Biology which you can use to further make yourself better in Biology.

Where can I download latest CBSE HOTS for Class 12 Biology Chapter 11 Biotechnology Principles and Processes

You can download the CBSE HOTS for Class 12 Biology Chapter 11 Biotechnology Principles and Processes for latest session from StudiesToday.com

Can I download the HOTS of Chapter 11 Biotechnology Principles and Processes Class 12 Biology in Pdf

Yes, you can click on the link above and download topic wise HOTS Questions Pdfs for Chapter 11 Biotechnology Principles and Processes Class 12 for Biology

Are the Class 12 Biology Chapter 11 Biotechnology Principles and Processes HOTS available for the latest session

Yes, the HOTS issued by CBSE for Class 12 Biology Chapter 11 Biotechnology Principles and Processes have been made available here for latest academic session

How can I download the Class 12 Biology Chapter 11 Biotechnology Principles and Processes HOTS

You can easily access the link above and download the Class 12 HOTS Biology Chapter 11 Biotechnology Principles and Processes for each topic

Is there any charge for the HOTS with solutions for Chapter 11 Biotechnology Principles and Processes Class 12 Biology

There is no charge for the HOTS and their answers for Chapter 11 Biotechnology Principles and Processes Class 12 CBSE Biology you can download everything free

What does HOTS stand for in Class 12 Biology Chapter 11 Biotechnology Principles and Processes

HOTS stands for "Higher Order Thinking Skills" in Chapter 11 Biotechnology Principles and Processes Class 12 Biology. It refers to questions that require critical thinking, analysis, and application of knowledge

How can I improve my HOTS in Class 12 Biology Chapter 11 Biotechnology Principles and Processes

Regular revision of HOTS given on studiestoday for Class 12 subject Biology Chapter 11 Biotechnology Principles and Processes can help you to score better marks in exams

Are HOTS questions important for Chapter 11 Biotechnology Principles and Processes Class 12 Biology exams

Yes, HOTS questions are important for Chapter 11 Biotechnology Principles and Processes Class 12 Biology exams as it helps to assess your ability to think critically, apply concepts, and display understanding of the subject.