CBSE Class 12 Biotechnology Syllabus

CLASS XII (2023-24)
COURSE- STRUCTURE- (THEORY)
One Paper                                                      Max. Marks 70+30
Time: 3 hrs.

Units                                                                    Marks
Unit V     Protein and Gene Manipulation                40
Unit VI    Cell Culture and Genetic Manipulation     30
              Practicals                                                   30
              Total                                                         100

One paper Time: 3 hrs.
Total Marks: 70

Unit-V Protein and Gene Manipulation 40 Marks
Chapter-1: Recombinant DNA Technology
Introduction, Tool of Recombinant DNA technology, Making rDNA molecule, Introduction of recombinant DNA into host cells, Identification of recombinants, Polymerase Chain Reaction (PCR), DNA Sequencing.
Chapter-2: Protein Structure and Engineering
Introduction to the world of proteins, Structure-function Relationship in proteins, Characterization of proteins, Protein based products, Designing proteins (Protein Engineering)
Chapter-3: Genomics, Proteomics and Bioinformatics
Gene prediction and counting, Genome similarity, SNPs and Comparative genomics, Functional genomics, Proteomics, Information sources, Analysis using bioinformatics tools.

Unit-VI Cell Culture and Genetic Manipulation    30 Marks
Chapter-1: Microbial Cell Culture and its Applications
Introduction, Microbial nutrition and culture techniques, Measurement and kinetics of microbial growth, Isolation of microbial products, Strain isolation and improvement, Applications of microbial culture technology.

Chapter -2: Plant Cell Culture and Applications
Introduction, Cell and tissue culture techniques, Applications of cell and tissue culture, Transgenic plants with beneficial traits, Biosafety of transgenic plants

Chapter-3: Animal Cell Culture and Applications
Introduction, Animal cell culture techniques, Applications of animal cell culture, Stem cell technology.

PRACTICALS 30 Marks
Note: Every student will be required to do the following experiments during the academic session.
1. Use of special equipment in biotechnology experiments
2. Isolation of bacterial plasmid DNA
3. Detection of DNA by gel electrophoresis
4. Estimation of DNA by UV spectroscopy
5. Isolation of bacteria from curd & staining of bacteria
6. Cell viability assay using Evan’s blue dye exclusion method
7. Data retrieval and database search using internet site NCBI and download a DNAand protein sequence from internet, analyze it and comment on it
8. Reading of a DNA sequencing gel to arrive at the sequence
9. Project work

Time: 3 Hours Max.               Marks 30
The scheme of evaluation at the end of the session will be as under:
A     Two experiments              6+6 (only one computer based practical)
         Practical record                 04
         Viva on Practical               04
B      Project work
        Write up                             05
        Viva on project                  05
          Total                               30

Note:- More emphasis should be given on hands on work in projects.

Prescribed Books:
1. A Text Book of Biotechnology - Class XI : Published by CBSE, New Delhi
2. As reference- Biotechnology - Class XI : Published by NCERT, New Delhi
3. A Laboratory Manual of Biotechnology - Class XI : Published by CBSE, New Delhi
4. A Text Book of Biotechnology - Class XII : Published by CBSE, New Delhi
5. A Laboratory Manual of Biotechnology - Class XII : Published by CBSE, New Delhi

Assessment Areas (Theory) 2023-24 Class XII
Biotechnology (045)    
Time : 3 hrs.                                  Maximum Marks: 70 Marks
Competencies Demonstrate, Knowledge and Understanding    50%
Application of Knowledge / Concepts    30%
Analyse, Evaluate and Create              20%

Note:
- Typology of questions: VSA including MCQs, Assertion – Reasoning type questions; SA; LA-I; LA-II; Source-based/ Case-based/ Passage-based/ Integrated assessment questions.
- An internal choice of approximately 33% would be provided.

Suggestive verbs for various competencies
- Demonstrate, Knowledge and Understanding
State, name, list, identify, define, suggest, describe, outline, summarize, etc.
- Application of Knowledge/Concepts
Calculate, illustrate, show, adapt, explain, distinguish, etc.
- Analyze, Evaluate and Create
Interpret, analyse, compare, contrast, examine, evaluate, discuss, construct, etc.