CBSE Class 12 Biology Biotechnology and Its Applications MCQs Set E

Question. Consumption of which one of the following foods can prevent the kind of blindness associated with vitamin ‘A’ deficiency?
(a) ‘Flavr Savr’ tomato
(b) Canolla
(c) Golden rice
(d) Bt-Brinjal

Answer: C

Question. The genetically-modified (GM) brinjal in India has been developed for
(a) insect-resistance
(b) enhancing shelf life
(c) enhancing mineral content
(d) drought-resistance

Answer: A

Question. Cry protein is obtained from
(a) Bacillus thuringiensis
(b) Bacillus subtilis
(c) Clostridium welchi
(d) E. coli

Answer: A

Question. Find out the incorrect statement.
(a) Human protein used to treat emphysema is a-1 antitrypsin.
(b) Human insulin is being commercially produced from a transgenic species of Agrobacterium tumefaciens.
(c) Rosie, the first transgenic cow, produced human protein enriched milk.
(d) Cry I Ab endotoxins obtained from Bacillus thuringiensis is effective against corn borers.

Answer: B

Question. Biopiracy is related to
(a) bioresearches
(b) traditional knowledge
(c) biomolecules and genes discovered
(d) all of the above

Answer: D

Question. Which step of Government of India has taken to cater requirement of patent terms and other emergency provisions in this regard ?
(a) Biopiracy act
(b) Indian patents bill
(c) RTI act
(d) Negotiable instruments act

Answer: B

Question. Select the correct statement.
(a) Genetic engineering works only on animals and has not yet been successfully used on plants.
(b) There are no risks associated with DNA technology.
(c) The first step in PCR is heat which is used to separate both the strands of target DNA.
(d) DNA from one organism will not bond to DNA from another animal.

Answer: C

Question. Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produced (in the host cells)
(a) both sense and anti-sense RNA.
(b) a particular hormone.
(c) an antifeedant.
(d) a toxic protein.

Answer: A

Question. The first human drug made using recombinant DNA technology was
(a) glyphosatase
(b) TPA
(c) insulin
(d) erythropoietin

Answer: C

Question. The first clinical gene therapy was given in 1990 to a 4 years old girl with enzyme deficiency of
(a) adenosine deaminase (ADA)
(b) tyrosine oxidase
(c) monamine oxidase
(d) glutamate dehydrogenase

Answer: A

Question. Read the following four statements (i-iv) and answer the question ?
(i) The first transgenic buffalo, Rosie produced milk which was human alpha-lactal albumin enriched.
(ii) Restriction enzymes are used in isolation of DNA from other macro-molecules.
(iii) Downstream processing is one of the steps of RDNA technology.
(iv) Disarmed pathogen vectors are also used in transfer of R-DNA into the host.
Which are the two statements having mistakes?
(a) (ii) and (iii)
(b) (iii) and (iv)
(c) (i) and (iii)
(d) (i) and (ii)

Answer: D

Question. Which one of the following statement is correct?
(a) The proteins encoded by the genes cry I Ac and cry II Ab control cotton bollworms.
(b) Protein encoded by cry I Ab controls corn borer.
(c) Both (a) and (b)
(d) Proteins encoded by cryI Ac and cry I Ab control flies.

Answer: C

Question. Which of the following statement(s) is/are correct?
(a) The procedure for chemical safety testing / toxicity is the same as that used for testing toxicity of drugs.
(b) Transgenic animals are more sensitive to the toxic substances than non-transgenic animals.
(c) Golden rice, a genetically engineered rice has high vitamin A (retinol) content.
(d) All of the above

Answer: D

Question. Bt toxin is harmful to insects like
(a) lepidoterans (tobacco budworm, armyworms)
(b) coleopterans (beetles)
(c) dipterans (flies and mosquito)
(d) all of the above

Answer: D

Question. The site of production of ADA in the body is
(a) bone marrow
(b) lymphocytes
(c) blood plasma
(d) monocytes

Answer: B

Question. How many varieties of rice has been estimated to be present in India?
(a) 2,000
(b) 20,000
(c) 200,000
(d) 2,000,000

Answer: C

Question. DNA or RNA segment tagged with a radioactive molecule is called ___________ .
(a) vector
(b) probe
(c) clone
(d) plasmid

Answer: B

Question. Today, transgenic models exist for many human diseases which includes
(i) Cancer
(ii) Cystic fibrosis
(iii) Rheumatoid arthritis
(iv) Alzhiemer’s disease
(a) (i) and (iii) only
(b) (ii) and (iii) only
(c) (i), (ii) and (iii) only
(d) all of these

Answer: D

Question. Maximum number of existing transgenic animals is of
(a) fish
(b) mice
(c) cow
(d) pig

Answer: B

Question. Which of the following statement(s) is/are incorrect?
(i) Insulin was originally extracted from pancreas of slaughtered pigs and cattle.
(ii) Animal insulin is difficult to obtain.
(iii) Animal insulin is identical to human insulin.
(iv) Non-human insulin caused some patients to develop allergy.
(v) Recombinant insulin is actually obtained from E. coli in bacterial cell.
(a) Only (i) and (ii)
(b) Only (iii) and (iv)
(c) Only (iii)
(d) Only (v)

Answer: C

Question. When a patient with defective ADA was treated, which of the following steps was performed for gene therapy?
(i) Lymphocytes were obtained from the patients.
(ii) Lymphocytes are transferred to culture dishes.
(iii) Lymphocytes were transfected with normal ADA genes.
(iv) The transfected cells are returned to the patients.
(a) All of these
(b) (iii) and (iv)
(c) Only (iv)
(d) SCID cannot be treated

Answer: A

Assertion/Reason Type Questions

In the following questions, a statement of Assertion is followed by a statement of Reason.
(a) If both Assertion and Reason are true and the Reason is the correct explanation of the Assertion.
(b) If both Assertion and Reason are true but the Reason is not the correct explanation of the Assertion. 
(c) If Assertion is true but Reason is false.
(d) If both Assertion and Reason are false.

Question. Assertion : Blood clotting is prevented by Hirudin protein.
Reason : The gene encoding for the Hirudin protein is transferred into Brassica napus, where ccumulation of hirudin occurs in the seeds.

Answer: B

Question. Assertion : Flavr Savr tomato is transgenic tomato that is capable of remaining fresh and retaining the flavour for a longer time.
Reason : In this, production of pectin degrading Polygalactouronase is blocked.

Answer: A

Question. Assertion : The GEAC (Genetic Engineering Approval Committee) has been set up by the Indian Government.
Reason : Introduction of GMO could have unpredictable result in the ecosystem.

Answer: A