CBSE Class 9 Science Chapter 01 Exploration Entering The World Of Secondary Science MCQs Set 01

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Question: Robert Hooke's discovery of cells in 1665 was made possible by which key piece of equipment?
A. A self-built microscope with roughly 200-300X magnification
B. An electron microscope using a beam of electrons
C. A light microscope with 10X and 40X objective lenses
D. A simple convex lens held over a slide
Show Answer & Explanation

Answer: (A) A self-built microscope with roughly 200-300X magnification

Explanation:
Hooke built his own microscope capable of about 200 to 300 times magnification and used it to examine a thin slice of cork, where he noticed small box-like compartments he named cells. Electron microscopes came much later and were not available to him.

Question: Why can electron microscopes reveal cell structures that light microscopes cannot?
A. They use a beam of electrons instead of light, allowing imaging at the nanometre scale
B. They use a stronger convex lens combination than light microscopes
C. They magnify objects by shining ordinary light through a coloured filter
D. They rely on staining agents like safranin to enlarge structures
Show Answer & Explanation

Answer: (A) They use a beam of electrons instead of light, allowing imaging at the nanometre scale

Explanation:
The chapter explains that electron microscopes use electron beams rather than visible light, which lets scientists see details at the nanometre scale with far greater clarity than a light microscope allows.

Question: In the potato osmosis activity, why did the piece kept in plain water swell while the one in salt solution shrank?
A. Salt entered the cells in Beaker B, forcing water out mechanically
B. Water moved into the piece with less solute and out of the piece with more solute, both trying to equalise concentration
C. The potato in Beaker A absorbed sugar, causing it to swell
D. The cell wall dissolved in salt solution, causing shrinkage
Show Answer & Explanation

Answer: (B) Water moved into the piece with less solute and out of the piece with more solute, both trying to equalise concentration

Explanation:
Osmosis drives water from a dilute solution toward a concentrated one across the selectively permeable membrane until concentrations equalise. The potato in plain water gained water and swelled, while the one in salt solution lost water and shrank; the salt or sugar molecules themselves do not cross the membrane.

Question: What is the main reason cheek cells shrink noticeably in concentrated sugar solution while onion peel cells only shrink internally?
A. Cheek cells contain more mitochondria than onion cells
B. Onion cells lack a cell membrane, so they cannot lose water
C. Onion cells have a rigid cell wall that holds their outer shape even as the membrane pulls inward, but cheek cells have no such wall
D. Cheek cells have a thicker cell membrane that resists water loss
Show Answer & Explanation

Answer: (C) Onion cells have a rigid cell wall that holds their outer shape even as the membrane pulls inward, but cheek cells have no such wall

Explanation:
• Plant cells like onion peel have a rigid cell wall outside the membrane.
• When water leaves by osmosis, the membrane pulls away from the wall but the wall keeps the overall shape intact.
• Animal cells such as cheek cells have no cell wall, so the whole cell shrinks visibly.

Question: A cell placed in a solution where the surrounding solute concentration is lower than inside the cell is said to be in which type of solution?
A. Isotonic solution
B. Hypertonic solution
C. Hypotonic solution
D. Concentrated solution
Show Answer & Explanation

Answer: (C) Hypotonic solution

Explanation:
When the external solute concentration is lower than inside the cell, the solution is called hypotonic, and water tends to move into the cell under such conditions.

Question: Which feature best distinguishes a eukaryotic cell from a prokaryotic cell?
A. Presence of cytoplasm
B. Presence of a cell membrane
C. Presence of a well-defined, membrane-bound nucleus and organelles
D. Ability to carry genetic material
Show Answer & Explanation

Answer: (C) Presence of a well-defined, membrane-bound nucleus and organelles

Explanation:
Both cell types have cytoplasm, a membrane, and genetic material, but only eukaryotic cells possess a true nucleus enclosed by a membrane along with other membrane-bound organelles; prokaryotic cells instead have a nucleoid region without a surrounding membrane.

Question: Mature human red blood cells lose their nucleus during development. What advantage does this provide?
A. It allows them to divide more rapidly than other cells
B. It creates extra room for haemoglobin, increasing oxygen-carrying capacity
C. It lets them produce their own proteins independently
D. It protects them from being broken down by lysosomes
Show Answer & Explanation

Answer: (B) It creates extra room for haemoglobin, increasing oxygen-carrying capacity

Explanation:
Without a nucleus taking up space, mature RBCs have more room for haemoglobin, which boosts how much oxygen they can transport throughout the body. The trade-off is that they cannot repair or divide, giving them a short lifespan of about 120 days.

Question: The rough appearance of Rough Endoplasmic Reticulum under an electron microscope is due to which feature?
A. Folded cristae on its surface
B. Ribosomes attached to its outer surface
C. A double membrane structure
D. Stored fat droplets on its surface
Show Answer & Explanation

Answer: (B) Ribosomes attached to its outer surface

Explanation:
RER gets its studded, rough look from ribosomes attached along its surface, and this is where much of the cell's protein synthesis and secretion happens. Smooth ER, lacking ribosomes, appears smooth and instead handles lipid and hormone synthesis.

Question: Camillo Golgi's discovery of the Golgi apparatus in 1898 faced initial doubt mainly because of what limitation?
A. He had used the wrong staining chemical on the tissue
B. Early microscopes could not clearly resolve the thread-like structure he observed
C. He mistook mitochondria for the Golgi apparatus
D. The barn owl nerve cells he studied did not actually contain the organelle
Show Answer & Explanation

Answer: (B) Early microscopes could not clearly resolve the thread-like structure he observed

Explanation:
Golgi observed a thread-like network using special staining, but the microscopes of that era lacked the resolution to confirm it clearly, so many scientists remained skeptical until electron microscopes verified its existence decades later.

Question: Why do mitochondria and chloroplasts both contain their own DNA and ribosomes?
A. Because they were artificially engineered by early plant cells
B. Because this feature suggests an evolutionary link to ancient single-celled organisms like bacteria
C. Because they need to store genetic material separately from the nucleus for safety
D. Because they must produce lysosomal enzymes independently
Show Answer & Explanation

Answer: (B) Because this feature suggests an evolutionary link to ancient single-celled organisms like bacteria

Explanation:
The presence of their own DNA and ribosomes, allowing them to make some proteins independently, points to mitochondria and chloroplasts sharing an evolutionary history with certain bacteria, as suggested in the chapter.

Question: A plant leucoplast found in a potato tuber most likely stores which substance?
A. Chlorophyll for photosynthesis
B. Coloured pigments for attracting pollinators
C. Starch as a food reserve
D. Cellulose for structural support
Show Answer & Explanation

Answer: (C) Starch as a food reserve

Explanation:
Leucoplasts are colourless plastids that store food materials such as starch, oils, or proteins; potato and taro cells specifically use leucoplasts to store starch.

Question: What would most likely happen to a plant if its central vacuoles lost most of their stored water?
A. The plant cells would divide more rapidly by mitosis
B. The cells would lose internal pressure, becoming less firm and causing the plant to wilt
C. The chloroplasts inside the cells would stop making chlorophyll
D. The cell walls would dissolve completely
Show Answer & Explanation

Answer: (B) The cells would lose internal pressure, becoming less firm and causing the plant to wilt

Explanation:
The large central vacuole maintains internal pressure by holding water; when water is lost, this pressure drops, cells become less firm, and the plant shows visible wilting.

Question: How does meiosis differ from mitosis in terms of outcome?
A. Meiosis produces two identical daughter cells, while mitosis produces four
B. Mitosis produces four daughter cells with half the chromosome number, while meiosis produces two identical cells
C. Meiosis involves two rounds of division producing four daughter cells with half the chromosome number, while mitosis produces two genetically identical cells
D. Both processes produce identical outcomes but occur in different organs
Show Answer & Explanation

Answer: (C) Meiosis involves two rounds of division producing four daughter cells with half the chromosome number, while mitosis produces two genetically identical cells

Explanation:
• Mitosis is a single division producing two daughter cells genetically identical to the parent, used for growth and repair.
• Meiosis involves two successive divisions, producing four daughter cells each with half the chromosome number.
• Meiosis occurs only in reproductive organs and creates genetic variation, unlike mitosis.

Question: According to the chapter, what typically happens when errors occur during mitosis in the human body?
A. It leads to genetic disorders affecting only offspring
B. It causes reduced fertility in reproductive organs
C. It can result in uncontrolled cell division and tumour formation
D. It prevents the formation of gametes
Show Answer & Explanation

Answer: (C) It can result in uncontrolled cell division and tumour formation

Explanation:
Faulty mitosis can disrupt the normal controlled division process, leading to abnormal chromosome numbers and uncontrolled growth that forms tumours, whereas errors in meiosis are more linked to genetic disorders and fertility issues.

Question: The classical Cell Theory, developed through the combined work of three scientists, states that new cells arise only from pre-existing cells. Which scientist specifically contributed this particular idea?
A. Matthias Schleiden
B. Theodor Schwann
C. Rudolf Virchow
D. Robert Hooke
Show Answer & Explanation

Answer: (C) Rudolf Virchow

Explanation:
Schleiden showed plants are made of cells and Schwann showed the same for animals, but it was Rudolf Virchow who added the crucial idea that new cells form only from pre-existing cells, completing the classical Cell Theory.

Question: Why do researchers consider hot springs such as those in Puga Valley significant for studying the origin of life?
A. Because such extreme environments resemble early Earth conditions, and rapid mineral deposits there may have shielded organic molecules and helped form the first cell membranes
B. Because these hot springs contain fossilised remains of the very first cells that ever existed
C. Because such water bodies host the greatest variety of modern multicellular organisms
D. Because the intense heat there generates oxygen needed for early photosynthesis
Show Answer & Explanation

Answer: (A) Because such extreme environments resemble early Earth conditions, and rapid mineral deposits there may have shielded organic molecules and helped form the first cell membranes

Explanation:
• Puga Valley's hot springs stay near boiling point even in a cold climate, mimicking early Earth conditions from about 3.5 billion years ago
• Scientists found calcium carbonate forming rapidly around these springs
• These deposits may have protected fragile organic molecules and contributed to the first protective membrane

Question: In the context of using a microscope, what does the term 'limit of resolution' describe?
A. The smallest distance between two points at which they can still be seen as separate rather than blurring into one
B. The maximum size of an object that fits on a microscope's stage
C. The combined magnifying power of the eyepiece and objective lens
D. The precise thickness of glass required for a clear slide
Show Answer & Explanation

Answer: (A) The smallest distance between two points at which they can still be seen as separate rather than blurring into one

Explanation:
For the unaided human eye, this limit is about 0.1 mm when viewed from the near point of 25 cm; anything closer than that appears as a single blurred point rather than two distinct ones.

Question: During Activity 2.1, a student measures a circular field of view with a diameter of 6 mm and counts 30 onion peel cells lying across that diameter. What is the approximate real size of one cell?
A. 200 micrometres
B. 20 micrometres
C. 300 micrometres
D. 2000 micrometres
Show Answer & Explanation

Answer: (A) 200 micrometres

Explanation:
First convert 6 mm to micrometres, giving 6000 micrometres. Dividing this by the 30 cells counted along the diameter gives 6000/30 = 200 micrometres per cell.

Question: When a Rhoeo leaf peel is soaked in concentrated sugar solution, why does the cell's outer boundary keep its original shape even though the contents inside visibly shrink?
A. The rigid cell wall holds its outline firm while only the membrane and cytoplasm pull inward as water is lost
B. The central vacuole swells to fill the space created by water loss
C. The chloroplasts absorb the escaping water and expand instead
D. The nucleus enlarges to occupy the space left behind by shrinking cytoplasm
Show Answer & Explanation

Answer: (A) The rigid cell wall holds its outline firm while only the membrane and cytoplasm pull inward as water is lost

Explanation:
Because the cell wall is rigid, it does not collapse the way the flexible cell membrane does; as water leaves through osmosis, the membrane and cytoplasm pull away from the fixed wall, creating a visible gap while the outer shape of the cell stays intact.

Question: Which structure inside the nucleus is responsible for producing the subunits that later travel to the cytoplasm and assemble into ribosomes?
A. Nucleolus
B. Chromatin
C. Nuclear membrane
D. Nuclear pore
Show Answer & Explanation

Answer: (A) Nucleolus

Explanation:
The nucleolus is described as the dense round body within the nucleus where ribosomal subunits are made before they exit through nuclear pores and combine in the cytoplasm.

Question: At which point in a cell's life does its thread-like chromatin material become organised into distinctly visible, rod-shaped chromosomes?
A. Just before the cell is about to divide
B. Immediately after a new cell membrane forms
C. Only when the cell permanently stops all activity
D. Whenever ribosomal subunits are being made in the nucleolus
Show Answer & Explanation

Answer: (A) Just before the cell is about to divide

Explanation:
In a resting, non-dividing cell the genetic material stays loosely tangled as chromatin, but as division approaches, this material condenses and organises itself into the compact, rod-like chromosomes that can be seen clearly.

Question: According to the chapter, how does diffusion differ from osmosis?
A. Diffusion is the movement of particles from a higher to a lower concentration and can occur without any membrane, whereas osmosis is specifically the movement of water across a selectively permeable membrane
B. Diffusion happens only in liquids while osmosis happens only in gases
C. Diffusion needs energy from the cell while osmosis happens automatically without any concentration difference
D. Diffusion moves only solute particles while osmosis moves solute and water at the same rate
Show Answer & Explanation

Answer: (A) Diffusion is the movement of particles from a higher to a lower concentration and can occur without any membrane, whereas osmosis is specifically the movement of water across a selectively permeable membrane

Explanation:
Diffusion is a broader process describing particles spreading from where they are concentrated to where they are scarce, with or without a membrane present. Osmosis is a special case of this idea, restricted to water crossing a selectively permeable membrane, such as when root cells absorb water from soil.

Question: The chapter compares a cell to a tiny living factory. What idea does this comparison mainly convey?
A. Different organelles each carry out a specific job, such as building materials, clearing waste, or supplying energy, and together they keep the whole cell running
B. Cells manufacture goods that are sold commercially, much like an actual factory
C. A cell needs constant human supervision to carry out its internal functions
D. Every organelle in a cell performs an identical function so that there is always a backup
Show Answer & Explanation

Answer: (A) Different organelles each carry out a specific job, such as building materials, clearing waste, or supplying energy, and together they keep the whole cell running

Explanation:
• Organelles such as the nucleus, ER, Golgi apparatus, mitochondria and lysosomes each handle a distinct task
• These tasks range from making proteins to producing energy and clearing waste
• The factory comparison highlights how these separate jobs combine into one coordinated, functioning unit

Question: What central idea did the botanist Gottlieb Haberlandt put forward, which later became the foundation of plant tissue culture technology?
A. Even a fully mature, differentiated plant cell can be induced to grow into an entire new plant if given suitable nutrients and conditions
B. Only young meristematic cells located at root and shoot tips are capable of dividing and regenerating
C. Plant cells permanently lose their ability to change function once they mature
D. A cell must contain chloroplasts in order to regenerate into a new plant
Show Answer & Explanation

Answer: (A) Even a fully mature, differentiated plant cell can be induced to grow into an entire new plant if given suitable nutrients and conditions

Explanation:
Haberlandt proposed that any living plant cell, not just embryonic or meristematic ones, retains the ability to develop into a complete plant under the right conditions. This property, called totipotency, became the basis for growing whole plants from single cells in laboratories.

Question: What key conclusion did J. Craig Venter's team draw after inserting laboratory-synthesised DNA into a bacterial cell whose own DNA had been removed?
A. DNA governs and directs the structure and activities of a cell
B. A complete living cell can be built entirely from non-living chemicals without using any pre-existing cell parts
C. Mitochondria are not essential for a bacterial cell to survive
D. Ribosomes can be chemically manufactured completely independent of any living cell
Show Answer & Explanation

Answer: (A) DNA governs and directs the structure and activities of a cell

Explanation:
Once the synthetic DNA was placed inside the emptied bacterial cell, the cell began growing and dividing exactly as instructed by that new genetic material, showing that DNA controls what a cell becomes and does. Notably, the rest of the cell, such as its cytoplasm and membrane, still came from an already living cell, so this was not a fully synthetic cell created from scratch.

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